Measurement of adenosine triphosphate content in single red blood cells using the firefly bioluminescent reaction

R. K. Kostuk, A. G. Muhs, F. H. Kirkpatrick, C. W. Gabel

Research output: Contribution to journalArticle

Abstract

A unique optical instrument is described which uses the firefly bioluminescent reaction to measure adenosine triphosphate (ATP) levels in single red blood cells. The method allows chemical content level to be associated with individual cell features. The optical instrument consists of a phase contrast microscope to view cells, a pulsed argon-ion laser to rupture the cell membrane, and a photon counting system to measure the bioluminescent yield. The technique has been calibrated against a standard ATP measurement using bulk analysis methods. The ATP loss mechanism for blood cells in a controlled depletion experiment was also investigated.

Original languageEnglish (US)
Pages (from-to)1527-1532
Number of pages6
JournalApplied optics
Volume18
Issue number10
DOIs
StatePublished - May 15 1979
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Measurement of adenosine triphosphate content in single red blood cells using the firefly bioluminescent reaction'. Together they form a unique fingerprint.

  • Cite this