Abstract
We have developed an experimental system to measure the permeability of the cultured endothelial monolayer. The luminal-to-abluminal flux of 125I-albumin across cultured pulmonary endothelium was expressed as a clearance rate equal to the permeability-surface area product. After clearance rate measurement for a 30-min base-line period, a test agent was added to the luminal side, and the clearance rate was remeasured during a 30-min experimental period. In control studies the base-line clearance rate was 0.343 ± 0.017 μl/min. After correction for the diffusional resistances of the filter and unstirred layers, the calculated permeability of the endothelial monolayer was 1.2 x 10-5 cm/s. When culture medium was the test agent, the experimental clearance rate was unchanged from the base-line value. After addition of 4 mM oleic acid to the luminal chamber, the clearance rate was 0.528 ± 0.017 μl/min compared with a base-line value of 0.330 ± 0.008 μl/min (P < 0.005). This method allows the calculation of endothelial permeability with correction for unstirred layers and the use of each monolayer as its own control.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1076-1083 |
| Number of pages | 8 |
| Journal | Journal of Applied Physiology |
| Volume | 62 |
| Issue number | 3 |
| State | Published - 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Physiology
- Endocrinology
- Orthopedics and Sports Medicine
- Physical Therapy, Sports Therapy and Rehabilitation
Cite this
Measurement of albumin permeability across endothelial monolayers in vitro. / Cooper, J. A.; Del Vecchio, P. J.; Minnear, F. L.; Burhop, K. E.; Selig, W. M.; Garcia, Joe GN; Malik, A. B.
In: Journal of Applied Physiology, Vol. 62, No. 3, 1987, p. 1076-1083.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measurement of albumin permeability across endothelial monolayers in vitro
AU - Cooper, J. A.
AU - Del Vecchio, P. J.
AU - Minnear, F. L.
AU - Burhop, K. E.
AU - Selig, W. M.
AU - Garcia, Joe GN
AU - Malik, A. B.
PY - 1987
Y1 - 1987
N2 - We have developed an experimental system to measure the permeability of the cultured endothelial monolayer. The luminal-to-abluminal flux of 125I-albumin across cultured pulmonary endothelium was expressed as a clearance rate equal to the permeability-surface area product. After clearance rate measurement for a 30-min base-line period, a test agent was added to the luminal side, and the clearance rate was remeasured during a 30-min experimental period. In control studies the base-line clearance rate was 0.343 ± 0.017 μl/min. After correction for the diffusional resistances of the filter and unstirred layers, the calculated permeability of the endothelial monolayer was 1.2 x 10-5 cm/s. When culture medium was the test agent, the experimental clearance rate was unchanged from the base-line value. After addition of 4 mM oleic acid to the luminal chamber, the clearance rate was 0.528 ± 0.017 μl/min compared with a base-line value of 0.330 ± 0.008 μl/min (P < 0.005). This method allows the calculation of endothelial permeability with correction for unstirred layers and the use of each monolayer as its own control.
AB - We have developed an experimental system to measure the permeability of the cultured endothelial monolayer. The luminal-to-abluminal flux of 125I-albumin across cultured pulmonary endothelium was expressed as a clearance rate equal to the permeability-surface area product. After clearance rate measurement for a 30-min base-line period, a test agent was added to the luminal side, and the clearance rate was remeasured during a 30-min experimental period. In control studies the base-line clearance rate was 0.343 ± 0.017 μl/min. After correction for the diffusional resistances of the filter and unstirred layers, the calculated permeability of the endothelial monolayer was 1.2 x 10-5 cm/s. When culture medium was the test agent, the experimental clearance rate was unchanged from the base-line value. After addition of 4 mM oleic acid to the luminal chamber, the clearance rate was 0.528 ± 0.017 μl/min compared with a base-line value of 0.330 ± 0.008 μl/min (P < 0.005). This method allows the calculation of endothelial permeability with correction for unstirred layers and the use of each monolayer as its own control.
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M3 - Article
C2 - 3571065
AN - SCOPUS:0023223817
VL - 62
SP - 1076
EP - 1083
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 3
ER -