Measurement of chemopreventive efficacy in skin biopsies

P. Bozzo, D. S. Alberts, L. Vaught, V. D. Da Silva, D. Thompson, J. Warnecke, R. C. Miller, J. Einspahr, P. H. Bartels

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


OBJECTIVE: To explore methods suitable for quantitative assessment of the efficacy of chemopreventive intervention. STUDY DESIGN: High-resolution imagery of nuclei from the suprabasal and basal cell layers of sun-damaged skin were recorded. There were 10 cases. A shave biopsy was taken from an area of clearly evident solar keratosis before and after treatment with 2-difluoromethyl-dl-ornithine (DFMO) and from the colateral forearm, treated with a placebo. A number of karyometric variables were computed and combined to derive marker features that provided a numeric measure of the degree of nuclear deviation from normal. RESULTS: DFMO treatment was effective overall in reducing the degree of nuclear abnormality seen in the biopsies; in 8 of the 10 cases there was a significant improvement. The placebo-treated arm did not show a statistically different abnormality from the untreated arm. CONCLUSION: Karyometric analysis can provide numeric measures that allow documentation of statistically significant regression of actinic keratotic lesions following treatment with DFMO.

Original languageEnglish (US)
Pages (from-to)300-312
Number of pages13
JournalAnalytical and Quantitative Cytology and Histology
Issue number4
StatePublished - 2001


  • Actinic keratoses
  • Chemopreventive efficacy
  • Computer-assisted
  • DFMO
  • Difluoromethyl-dl-ornithine
  • Image analysis
  • Keratosis
  • Measures of lesion progression/regression

ASJC Scopus subject areas

  • Anatomy
  • Histology


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