Measurement of ion channel function using conventional patch-clamp techniques

Carmelle V. Remillard, Jason Yuan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Transmembrane ion flux plays an important role in the control of membrane potential (Em) and contractility of pulmonary vascular smooth muscle, the end effector in the regulation of pulmonary vasomotor activity. Ion channels are integral membrane proteins possessing selective permeability for monovalent and divalent ions. The activity of ion channels is dependent on their biophysical characteristics, molecular composition, and physiological function. This chapter confronts the more practical aspects of determining ion channel activity and expression, including the direct measurement of transmembrane current flow in intact cells.

Original languageEnglish (US)
Title of host publicationIon Channels in the Pulmonary Vasculature
PublisherCRC Press
Pages615-634
Number of pages20
ISBN (Electronic)9780849350375
ISBN (Print)0824759680, 9780824759681
StatePublished - Jan 1 2005
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Remillard, C. V., & Yuan, J. (2005). Measurement of ion channel function using conventional patch-clamp techniques. In Ion Channels in the Pulmonary Vasculature (pp. 615-634). CRC Press.