Measurement of normal and anomalous diffusion of dyes within protein structures fabricated via multiphoton excited cross-linking

Swarna Basu, Charles William Wolgemuth, Paul J. Campagnola

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We demonstrate microscale spatial and chemical control of diffusion within protein matrixes created through the use of nonlinear multiphoton excited photochemistry. The mobility of fluorescent dyes of different mass and composition within controlled cross-linked environments has been measured using two-photon excited fluorescence recovery after photobleaching (FRAP). The diffusion times for several rhodamine and sulforhodamine dyes within these fabricated structures were found to be approximately 3-4 orders of magnitude slower than in free solution. The precise diffusion times can be tuned by varying the laser exposure during the fabrication of the matrix, and the diffusion can be correlated with the mesh size determined by TEM and Flory-Rehner analysis. We find that the hydrophobic Texas Red dyes (sulforhodamines) exhibit diffusion that is highly anomalous, indicative of a strong interaction with the hydrophobic cross-linked protein matrix. These results suggests the use of these cross-linked protein matrixes as ideal model systems in which to systematically study anomalous diffusion. Finally, the diffusion can be tuned within a multilayered protein matrix, and this in conjunction with slow diffusion also suggests the use of these structures in controlled release applications.

Original languageEnglish (US)
Pages (from-to)2347-2357
Number of pages11
JournalBiomacromolecules
Volume5
Issue number6
DOIs
StatePublished - Nov 2004
Externally publishedYes

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Coloring Agents
Dyes
Proteins
Fluorescence Recovery After Photobleaching
Photochemistry
Photobleaching
Rhodamines
Photochemical reactions
Hydrophobic and Hydrophilic Interactions
Fluorescent Dyes
Photons
Lasers
Fluorescence
Transmission electron microscopy
Fabrication
Recovery
Chemical analysis

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Measurement of normal and anomalous diffusion of dyes within protein structures fabricated via multiphoton excited cross-linking. / Basu, Swarna; Wolgemuth, Charles William; Campagnola, Paul J.

In: Biomacromolecules, Vol. 5, No. 6, 11.2004, p. 2347-2357.

Research output: Contribution to journalArticle

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