Measuring bacterial contaminants in ultrapure water. A rapid analytical method

Ian L Pepper, K. L. Josephson, R. L. Bailey, M. D. Burr, S. D. Pillai, D. L. Tolliver, S. Pulido

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The availability of ultrapure water is one of the basic requirements of semiconductor manufacturing. The methods currently used to detect microbial contaminants in UP water can significantly underestimate the actual levels of contamination. This article describes the use of a specialized molecular biology technique known as the polymerase chain reaction that provides rapid and sensitive detection of microbial contaminants. By employing this technique on UP-water samples collected over an 11-month period, we demonstrated that microbial contamination was episodic in nature and that 44.1% of the samples tested showed a positive indication of bacterial contamination of at least 1 CFU/L. The attractive features of this technique are its sensitivity, cost-effectiveness, and speed of detection.

Original languageEnglish (US)
JournalMicrocontamination
Volume12
Issue number10
StatePublished - Oct 1994

Fingerprint

contaminants
contamination
Contamination
Impurities
Water
water
molecular biology
polymerase chain reaction
cost effectiveness
Molecular biology
Polymerase chain reaction
Cost effectiveness
availability
indication
manufacturing
Availability
Semiconductor materials
requirements
sensitivity

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Pepper, I. L., Josephson, K. L., Bailey, R. L., Burr, M. D., Pillai, S. D., Tolliver, D. L., & Pulido, S. (1994). Measuring bacterial contaminants in ultrapure water. A rapid analytical method. Microcontamination, 12(10).

Measuring bacterial contaminants in ultrapure water. A rapid analytical method. / Pepper, Ian L; Josephson, K. L.; Bailey, R. L.; Burr, M. D.; Pillai, S. D.; Tolliver, D. L.; Pulido, S.

In: Microcontamination, Vol. 12, No. 10, 10.1994.

Research output: Contribution to journalArticle

Pepper, IL, Josephson, KL, Bailey, RL, Burr, MD, Pillai, SD, Tolliver, DL & Pulido, S 1994, 'Measuring bacterial contaminants in ultrapure water. A rapid analytical method', Microcontamination, vol. 12, no. 10.
Pepper IL, Josephson KL, Bailey RL, Burr MD, Pillai SD, Tolliver DL et al. Measuring bacterial contaminants in ultrapure water. A rapid analytical method. Microcontamination. 1994 Oct;12(10).
Pepper, Ian L ; Josephson, K. L. ; Bailey, R. L. ; Burr, M. D. ; Pillai, S. D. ; Tolliver, D. L. ; Pulido, S. / Measuring bacterial contaminants in ultrapure water. A rapid analytical method. In: Microcontamination. 1994 ; Vol. 12, No. 10.
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