Measuring oxygen tension modulation, induced by a new pre-radiotherapy therapeutic, in a mammary window chamber mouse model

Rachel Schafer, Arthur F. Gmitro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Tumor regions under hypoxic or low oxygen conditions respond less effectively to many treatment strategies, including radiation therapy. A novel investigational therapeutic, NVX-108 (NuvOx Pharma), has been developed to increase delivery of oxygen through the use of a nano-emulsion of dodecofluoropentane. By raising pO2 levels prior to delivering radiation, treatment efficacy may be improved. To aid in evaluating the novel drug, oxygen tension was quantitatively measured, spatially and temporally, to record the effect of administrating NVX-108 in an orthotopic mammary window chamber mouse model of breast cancer. The oxygen tension was measured through the use of an oxygen-sensitive coating, comprised of phosphorescent platinum porphyrin dye embedded in a polystyrene matrix. The coating, applied to the surface of the coverslip of the window chamber through spin coating, is placed in contact with the mammary fat pad to record the oxygenation status of the surface tissue layer. Prior to implantation of the window chamber, a tumor is grown in the SCID mouse model by injection of MCF-7 cells into the mammary fat pad. Two-dimensional spatial distributions of the pO2 levels were obtained through conversion of measured maps of phosphorescent lifetime. The resulting information on the spatial and temporal variation of the induced oxygen modulation could provide valuable insight into the optimal timing between administration of NVX-108 and radiation treatment to provide the most effective treatment outcome.

Original languageEnglish (US)
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
EditorsDaniel L. Farkas, Dan V. Nicolau, Robert C. Leif
PublisherSPIE
ISBN (Electronic)9781628414189
DOIs
StatePublished - Jan 1 2015
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII - San Francisco, United States
Duration: Feb 9 2015Feb 11 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9328
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
CountryUnited States
CitySan Francisco
Period2/9/152/11/15

Keywords

  • Cancer microenvironment
  • Oxygen Sensing
  • Phosphorescent Lifetime
  • Window Chamber

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Schafer, R., & Gmitro, A. F. (2015). Measuring oxygen tension modulation, induced by a new pre-radiotherapy therapeutic, in a mammary window chamber mouse model. In D. L. Farkas, D. V. Nicolau, & R. C. Leif (Eds.), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII [932817] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9328). SPIE. https://doi.org/10.1117/12.2080236