Mechanical manipulation of single titin molecules with laser tweezers

M. S Z Kellermayer, S. Smith, C. Bustamante, Hendrikus "Henk" Granzier, Trinick, Bullard, Greaser, Linke, Pollack, TerKeurs, Vigoreaux, Qian

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Titin (also known as connectin) is a giant filamentous polypeptide of multi-domain construction spanning between the Z- and M-lines of the vertebrate muscle sarcomere. The molecule is significant in maintaining sarcomeric structural integrity and generating passive muscle force via its elastic properties. Here we summarize our efforts to characterize titin's elastic properties by manipulating single molecules with force-measuring laser tweezers. The titin molecule can be described as an entropic spring in which domain unfolding occurs at high forces during stretch and refolding at low forces during release. Statistical analysis of a large number (>500) of stretch-release experiments and comparison of experimental data with the predictions of the wormlike chain theory permit the estimation of unfolded titin's mean persistence length as 16.86 Å (±0.11 SD). The slow rates of unfolding and refolding compared with the rates of stretch and release, respectively, result in a state of non-equilibrium and the display of force hysteresis. Folding kinetics as the source of non-equilibrium is directly demonstrated here by the abolishment of force hysteresis in the presence of chemical denaturant. Experimental observations were well simulated by superimposing a simple domain folding kinetics model on the wormlike chain behavior of titin and considering the characteristics of the compliant laser trap. The original video presentation of this paper may be viewed on the web at http://www.pote.hu/mm/prezentacio/mkpres/mkpres.htm.

Original languageEnglish (US)
Pages (from-to)111-128
Number of pages18
JournalAdvances in Experimental Medicine and Biology
Volume481
StatePublished - 2000
Externally publishedYes

Fingerprint

Optical Tweezers
Connectin
Molecules
Lasers
Muscle
Hysteresis
Muscles
Sarcomeres
Kinetics
Structural integrity
Vertebrates
Statistical methods
Display devices
Peptides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kellermayer, M. S. Z., Smith, S., Bustamante, C., Granzier, H. H., Trinick, Bullard, ... Qian (2000). Mechanical manipulation of single titin molecules with laser tweezers. Advances in Experimental Medicine and Biology, 481, 111-128.

Mechanical manipulation of single titin molecules with laser tweezers. / Kellermayer, M. S Z; Smith, S.; Bustamante, C.; Granzier, Hendrikus "Henk"; Trinick; Bullard; Greaser; Linke; Pollack; TerKeurs; Vigoreaux; Qian.

In: Advances in Experimental Medicine and Biology, Vol. 481, 2000, p. 111-128.

Research output: Contribution to journalArticle

Kellermayer, MSZ, Smith, S, Bustamante, C, Granzier, HH, Trinick, Bullard, Greaser, Linke, Pollack, TerKeurs, Vigoreaux & Qian 2000, 'Mechanical manipulation of single titin molecules with laser tweezers', Advances in Experimental Medicine and Biology, vol. 481, pp. 111-128.
Kellermayer MSZ, Smith S, Bustamante C, Granzier HH, Trinick, Bullard et al. Mechanical manipulation of single titin molecules with laser tweezers. Advances in Experimental Medicine and Biology. 2000;481:111-128.
Kellermayer, M. S Z ; Smith, S. ; Bustamante, C. ; Granzier, Hendrikus "Henk" ; Trinick ; Bullard ; Greaser ; Linke ; Pollack ; TerKeurs ; Vigoreaux ; Qian. / Mechanical manipulation of single titin molecules with laser tweezers. In: Advances in Experimental Medicine and Biology. 2000 ; Vol. 481. pp. 111-128.
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