Mechanical property quantification of endothelial cells using scanning acoustic microscopy

A. Shelke, S. Brand, Tribikram Kundu, J. Bereiter-Hahn, C. Blasé

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

The mechanical properties of cells reflect dynamic changes of cellular organization which occur during physiologic activities like cell movement, cell volume regulation or cell division. Thus the study of cell mechanical properties can yield important information for understanding these physiologic activities. Endothelial cells form the thin inner lining of blood vessels in the cardiovascular system and are thus exposed to shear stress as well as tensile stress caused by the pulsatile blood flow. Endothelial dysfunction might occur due to reduced resistance to mechanical stress and is an initial step in the development of cardiovascular disease like, e.g., atherosclerosis. Therefore we investigated the mechanical properties of primary human endothelial cells (HUVEC) of different age using scanning acoustic microscopy at 1.2 GHz. The HUVECs are classified as young (t D < 90 h) and old (t D > 90 h) cells depending upon the generation time for the population doubling of the culture (t D). Longitudinal sound velocity and geometrical properties of cells (thickness) were determined using the material signature curve V(z) method for variable culture condition along spatial coordinates. The plane wave technique with normal incidence is assumed to solve two-dimensional wave equation. The size of the cells is modeled using multilayered (solid-fluid) system. The propagation of transversal wave and surface acoustic wave are neglected in soft matter analysis. The biomechanical properties of HUVEC cells are quantified in an age dependent manner.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Volume8348
DOIs
StatePublished - 2012
EventHealth Monitoring of Structural and Biological Systems 2012 - San Diego, CA, United States
Duration: Mar 12 2012Mar 15 2012

Other

OtherHealth Monitoring of Structural and Biological Systems 2012
CountryUnited States
CitySan Diego, CA
Period3/12/123/15/12

Fingerprint

Endothelial Cells
Endothelial cells
Microscopy
Quantification
Mechanical Properties
Scanning
Acoustics
mechanical properties
microscopy
Mechanical properties
scanning
acoustics
Cell
cells
Cells
Cardiovascular system
Blood vessels
Acoustic wave velocity
Wave equations
Linings

Keywords

  • Acoustic microscopy
  • Cell aging
  • HUVEC
  • Material signature curve
  • Plane wave theory

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Shelke, A., Brand, S., Kundu, T., Bereiter-Hahn, J., & Blasé, C. (2012). Mechanical property quantification of endothelial cells using scanning acoustic microscopy. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 8348). [83481T] https://doi.org/10.1117/12.917512

Mechanical property quantification of endothelial cells using scanning acoustic microscopy. / Shelke, A.; Brand, S.; Kundu, Tribikram; Bereiter-Hahn, J.; Blasé, C.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 8348 2012. 83481T.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shelke, A, Brand, S, Kundu, T, Bereiter-Hahn, J & Blasé, C 2012, Mechanical property quantification of endothelial cells using scanning acoustic microscopy. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 8348, 83481T, Health Monitoring of Structural and Biological Systems 2012, San Diego, CA, United States, 3/12/12. https://doi.org/10.1117/12.917512
Shelke A, Brand S, Kundu T, Bereiter-Hahn J, Blasé C. Mechanical property quantification of endothelial cells using scanning acoustic microscopy. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 8348. 2012. 83481T https://doi.org/10.1117/12.917512
Shelke, A. ; Brand, S. ; Kundu, Tribikram ; Bereiter-Hahn, J. ; Blasé, C. / Mechanical property quantification of endothelial cells using scanning acoustic microscopy. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 8348 2012.
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