Mechanical response and conformational amplification in α-Helical coiled coils

Osman N. Yogurtcu, Charles William Wolgemuth, Sean X. Sun

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

α-Helical coiled coils (CCs) are ubiquitous tertiary structural domains that are often found in mechanoproteins. CCs have mechanical rigidity and are often involved in force transmission between protein domains. Although crystal structures of CCs are available, information about their conformational flexibility is limited. The role of hydrophobic interactions in determining the CC conformation is not clear. In this work we examined the mechanical responses of typical CCs and constructed a coarse-grained mechanical model to describe the conformation of the protein. The model treats α-helices as elastic rods. Hydrophobic bonds arranged in a repeated pattern determine the CC structure. The model is compared with moleculardynamics simulations of CCs under force. We also estimate the effective bending and twisting persistence length of the CC. The model allows us to examine unconventional responses of the CC, including significant conformational amplification upon binding of a small molecule. We find that the CC does not behave as a simple elastic rod and shows complex nonlinear responses. These results are significant for understanding the role of CC structures in chemoreceptors, motor proteins, and mechanotransduction in general.

Original languageEnglish (US)
Pages (from-to)3895-3904
Number of pages10
JournalBiophysical Journal
Volume99
Issue number12
DOIs
StatePublished - Dec 15 2010
Externally publishedYes

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Protein Conformation
Hydrophobic and Hydrophilic Interactions
Proteins
Protein Domains

ASJC Scopus subject areas

  • Biophysics

Cite this

Mechanical response and conformational amplification in α-Helical coiled coils. / Yogurtcu, Osman N.; Wolgemuth, Charles William; Sun, Sean X.

In: Biophysical Journal, Vol. 99, No. 12, 15.12.2010, p. 3895-3904.

Research output: Contribution to journalArticle

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