Mechanical stretch induces activation of skeletal muscle satellite cells in vitro

R. Tatsumi, S. M. Sheehan, H. Iwasaki, A. Hattori, Ronald E Allen

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Cultured quiescent satellite cells were subjected to mechanical stretch in a FlexerCell System. In response to stretch, satellite cells entered the cell cycle earlier than if they were under control conditions. Only a brief period of stretch, as short as 2 h, was necessary to stimulate activation. Additionally, conditioned medium from stretched cells could activate unstretched satellite cells. The presence of HGF on c-met-positive myogenic cells was detected by immunofluorescence at 12 h in culture, and immunoblots demonstrated that HGF was released by stretched satellite cells into medium. Also, stretch activation could be abolished by the addition of anti-HGF antibodies to stretched cultures, and activity in conditioned medium from stretched cells could be neutralized by anti-HGF antibodies. In addition, stretch appeared to cause release of preexisting HGF from the extracellular matrix. These experiments suggest that HGF may be involved in linking mechanical perturbation of muscle to satellite cell activation.

Original languageEnglish (US)
Pages (from-to)107-114
Number of pages8
JournalExperimental Cell Research
Volume267
Issue number1
DOIs
StatePublished - Jul 1 2001

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Skeletal Muscle Satellite Cells
Conditioned Culture Medium
Anti-Idiotypic Antibodies
In Vitro Techniques
Fluorescent Antibody Technique
Extracellular Matrix
Cell Cycle
Muscles

Keywords

  • c-met
  • HGF
  • Muscle
  • Regeneration
  • Satellite cell

ASJC Scopus subject areas

  • Cell Biology

Cite this

Mechanical stretch induces activation of skeletal muscle satellite cells in vitro. / Tatsumi, R.; Sheehan, S. M.; Iwasaki, H.; Hattori, A.; Allen, Ronald E.

In: Experimental Cell Research, Vol. 267, No. 1, 01.07.2001, p. 107-114.

Research output: Contribution to journalArticle

Tatsumi, R. ; Sheehan, S. M. ; Iwasaki, H. ; Hattori, A. ; Allen, Ronald E. / Mechanical stretch induces activation of skeletal muscle satellite cells in vitro. In: Experimental Cell Research. 2001 ; Vol. 267, No. 1. pp. 107-114.
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