Mechanically induced intercellular calcium communication in confined endothelial structures

Michael Junkin, Yi Lu, Juexuan Long, Pierre A Deymier, James B. Hoying, Pak Kin Wong

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Calcium signaling in the diverse vascular structures is regulated by a wide range of mechanical and biochemical factors to maintain essential physiological functions of the vasculature. To properly transmit information, the intercellular calcium communication mechanism must be robust against various conditions in the cellular microenvironment. Using plasma lithography geometric confinement, we investigate mechanically induced calcium wave propagation in networks of human umbilical vein endothelial cells organized. Endothelial cell networks with confined architectures were stimulated at the single cell level, including using capacitive force probes. Calcium wave propagation in the network was observed using fluorescence calcium imaging. We show that mechanically induced calcium signaling in the endothelial networks is dynamically regulated against a wide range of probing forces and repeated stimulations. The calcium wave is able to propagate consistently in various dimensions from monolayers to individual cell chains, and in different topologies from linear patterns to cell junctions. Our results reveal that calcium signaling provides a robust mechanism for cell-cell communication in networks of endothelial cells despite the diversity of the microenvironmental inputs and complexity of vascular structures.

Original languageEnglish (US)
Pages (from-to)2049-2056
Number of pages8
JournalBiomaterials
Volume34
Issue number8
DOIs
StatePublished - Mar 2013

Fingerprint

Calcium Signaling
Calcium
Communication
Endothelial cells
Blood Vessels
Endothelial Cells
Wave propagation
Cellular Microenvironment
Intercellular Junctions
Optical Imaging
Human Umbilical Vein Endothelial Cells
Plasma confinement
Cell Communication
Lithography
Monolayers
Fluorescence
Topology
Plasmas
Imaging techniques

Keywords

  • Calcium
  • Cell signaling
  • Endothelial cell
  • Micropatterning
  • Plasma lithography

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Mechanically induced intercellular calcium communication in confined endothelial structures. / Junkin, Michael; Lu, Yi; Long, Juexuan; Deymier, Pierre A; Hoying, James B.; Wong, Pak Kin.

In: Biomaterials, Vol. 34, No. 8, 03.2013, p. 2049-2056.

Research output: Contribution to journalArticle

Junkin, Michael ; Lu, Yi ; Long, Juexuan ; Deymier, Pierre A ; Hoying, James B. ; Wong, Pak Kin. / Mechanically induced intercellular calcium communication in confined endothelial structures. In: Biomaterials. 2013 ; Vol. 34, No. 8. pp. 2049-2056.
@article{10ec42033c9a42ddb910cc221a7b1066,
title = "Mechanically induced intercellular calcium communication in confined endothelial structures",
abstract = "Calcium signaling in the diverse vascular structures is regulated by a wide range of mechanical and biochemical factors to maintain essential physiological functions of the vasculature. To properly transmit information, the intercellular calcium communication mechanism must be robust against various conditions in the cellular microenvironment. Using plasma lithography geometric confinement, we investigate mechanically induced calcium wave propagation in networks of human umbilical vein endothelial cells organized. Endothelial cell networks with confined architectures were stimulated at the single cell level, including using capacitive force probes. Calcium wave propagation in the network was observed using fluorescence calcium imaging. We show that mechanically induced calcium signaling in the endothelial networks is dynamically regulated against a wide range of probing forces and repeated stimulations. The calcium wave is able to propagate consistently in various dimensions from monolayers to individual cell chains, and in different topologies from linear patterns to cell junctions. Our results reveal that calcium signaling provides a robust mechanism for cell-cell communication in networks of endothelial cells despite the diversity of the microenvironmental inputs and complexity of vascular structures.",
keywords = "Calcium, Cell signaling, Endothelial cell, Micropatterning, Plasma lithography",
author = "Michael Junkin and Yi Lu and Juexuan Long and Deymier, {Pierre A} and Hoying, {James B.} and Wong, {Pak Kin}",
year = "2013",
month = "3",
doi = "10.1016/j.biomaterials.2012.11.060",
language = "English (US)",
volume = "34",
pages = "2049--2056",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "8",

}

TY - JOUR

T1 - Mechanically induced intercellular calcium communication in confined endothelial structures

AU - Junkin, Michael

AU - Lu, Yi

AU - Long, Juexuan

AU - Deymier, Pierre A

AU - Hoying, James B.

AU - Wong, Pak Kin

PY - 2013/3

Y1 - 2013/3

N2 - Calcium signaling in the diverse vascular structures is regulated by a wide range of mechanical and biochemical factors to maintain essential physiological functions of the vasculature. To properly transmit information, the intercellular calcium communication mechanism must be robust against various conditions in the cellular microenvironment. Using plasma lithography geometric confinement, we investigate mechanically induced calcium wave propagation in networks of human umbilical vein endothelial cells organized. Endothelial cell networks with confined architectures were stimulated at the single cell level, including using capacitive force probes. Calcium wave propagation in the network was observed using fluorescence calcium imaging. We show that mechanically induced calcium signaling in the endothelial networks is dynamically regulated against a wide range of probing forces and repeated stimulations. The calcium wave is able to propagate consistently in various dimensions from monolayers to individual cell chains, and in different topologies from linear patterns to cell junctions. Our results reveal that calcium signaling provides a robust mechanism for cell-cell communication in networks of endothelial cells despite the diversity of the microenvironmental inputs and complexity of vascular structures.

AB - Calcium signaling in the diverse vascular structures is regulated by a wide range of mechanical and biochemical factors to maintain essential physiological functions of the vasculature. To properly transmit information, the intercellular calcium communication mechanism must be robust against various conditions in the cellular microenvironment. Using plasma lithography geometric confinement, we investigate mechanically induced calcium wave propagation in networks of human umbilical vein endothelial cells organized. Endothelial cell networks with confined architectures were stimulated at the single cell level, including using capacitive force probes. Calcium wave propagation in the network was observed using fluorescence calcium imaging. We show that mechanically induced calcium signaling in the endothelial networks is dynamically regulated against a wide range of probing forces and repeated stimulations. The calcium wave is able to propagate consistently in various dimensions from monolayers to individual cell chains, and in different topologies from linear patterns to cell junctions. Our results reveal that calcium signaling provides a robust mechanism for cell-cell communication in networks of endothelial cells despite the diversity of the microenvironmental inputs and complexity of vascular structures.

KW - Calcium

KW - Cell signaling

KW - Endothelial cell

KW - Micropatterning

KW - Plasma lithography

UR - http://www.scopus.com/inward/record.url?scp=84871494174&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871494174&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2012.11.060

DO - 10.1016/j.biomaterials.2012.11.060

M3 - Article

C2 - 23267827

AN - SCOPUS:84871494174

VL - 34

SP - 2049

EP - 2056

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 8

ER -