Mechanism of v-Src- and mitogen-activated protein kinase-induced reduction of gap junction communication

G. Trevor Cottrell, Rui Lin, Bonnie J. Warn-Cramer, Alan F. Lau, Janis M. Burt

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

Connexin (Cx)43 gap junction channels are phosphorylated by numerous protein kinases, with the net effect typically being a reduction in gap junction communication (GJC). This reduction must result from a decrease in channel open probability, unitary conductance, or permselectivity, because previous results suggest that channel number is unaffected. Coexpression of v-Src with wild-type Cx43 (Cx43-wt) but not Cx43 with tyrosine to phenylalanine substitutions at 247 and 265 (Cx43-Y247,265F) resulted in reduced electrical and dye coupling but no change in single-channel amplitudes. EGF treatment of cells expressing Cx43-wt but not Cx43 with serine to alanine substitutions at 255, 279, and 282 (Cx43-S255,279,282A) resulted in reduced GJC, also with no change in single-channel amplitude. Dye coupling was reduced to a far greater extent than electrical coupling, suggesting that channel selectivity was also altered but with minimal effect on unitary conductance. The absence of Src- and MAPK-induced reductions in single-channel amplitude suggests that the decreases in GJC induced by these kinases result from reduced channel open probability and possibly altered selectivity.

Original languageEnglish (US)
Pages (from-to)C511-C520
JournalAmerican Journal of Physiology - Cell Physiology
Volume284
Issue number2 53-2
DOIs
StatePublished - Feb 1 2003

Keywords

  • Connexins
  • Dye permeability
  • Electrophysiology
  • Epidermal growth factor
  • Growth factors

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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