Mechanisms underlying increased release of endothelin-1 from aorta in diabetic rats

Ayako Makino, Shu Ichi Oda, Katsuo Kamata

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

To clarify the mechanism underlying increased endothelin-1 release in diabetic rats, we examined its release from thoracic aortas obtained from streptozotocin-induced diabetic rats. The methoxamine-induced contraction was significantly inhibited by BQ-123 plus BQ-788 (specific antagonists for ETA and ETB receptors) in diabetic, but not control rats. Preincubation with phosphoramidon also inhibited the methoxamine-induced contraction in diabetic but not control rats. The expression of prepro endothelin-1 mRNA was significantly enhanced in aortas from streptozotocin-induced diabetic rats. These results suggest that the increases in the basal and α-agonist-induced release of endothelin-1 in the diabetic state may be due to an overexpression of the mRNA for prepro endothelin-1.

Original languageEnglish (US)
Pages (from-to)639-645
Number of pages7
JournalPeptides
Volume22
Issue number4
DOIs
StatePublished - 2001

Keywords

  • Diabetes
  • Endothelin-1
  • Methoxamine
  • PreproET-1 mRNA
  • Rats
  • Streptozotocin
  • Thoracic aorta

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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