Mechano-transduction and barrier regulation in lung microvascular endothelial cells

Kristina Giantsos, Mark Cluff, Randal Dull

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Alterations in endothelial permeability are a hallmark of inflammation as well as the underlying cause of many clinical syndromes. Quantifying changes in endothelial barrier properties to water and macromolecules can be an important means of assessing the degree of cellular injury and, conversely, the effect of therapies to attenuate the inflammatory cascade. We use a combination of an isolated organ system and two cell culture models to investigate mechanisms of endothelial barrier regulation under variety of experimental conditions. Each assay has its own experimental strengths and limitations and must be used appropriately for the questions being asked. When used collectively, they can provide significant insight into the molecular regulation of lung endothelial permeability.

Original languageEnglish (US)
Title of host publicationPermeability Barrier
Subtitle of host publicationMethods and Protocols
EditorsKursad Turksen
Pages291-301
Number of pages11
DOIs
StatePublished - Oct 24 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume763
ISSN (Print)1064-3745

Keywords

  • Endothelium
  • Filtration coefficient
  • Hydraulic conductivity
  • Lung
  • Lung permeability -coefficient
  • Mechano-transduction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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