Mechanoporation induced by diffuse traumatic brain injury: An irreversible or reversible response to injury?

Orsolya Farkas, Jonathan Lifshitz, John T. Povlishock

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Diffuse traumatic brain injury (DTBI) is associated with neuronal plasmalemmal disruption, leading to either necrosis or reactive change without cell death. This study examined whether enduring membrane perturbation consistently occurs, leading to cell death, or if there is the potential for transient perturbation followed by resealing/recovery. We also examined the relationship of these events to calpain-mediated spectrin proteolysis (CMSP). To assess plasmalemmal disruption, rats (n = 21) received intracerebroventricular infusion 2 h before DTBI of a normally excluded 10 kDa fluorophore-labeled dextran. To reveal plasmalemmal resealing or enduring disruption, rats were infused with another labeled dextran 2 h (n = 10) or 6 h (n = 11) after injury. Immunohistochemistry for the 150 kDa spectrin breakdown product evaluated the concomitant role of CMSP. Neocortical neurons were followed with confocal and electron microscopy. After DTBI at 4 and 8 h, 55% of all tracer-flooded neurons contained both dextrans, demonstrating enduring plasmalemmal leakage, with many demonstrating necrosis. At 4 h, 12.0% and at 8 h, 15.7% of the dual tracer-flooded neurons showed CMSP, yet, these demonstrated less advanced cellular change. At 4 h, 39.0% and at 8 h, 24.4% of all tracer-flooded neurons revealed only preinjury dextran uptake, consistent with membrane resealing, whereas 7.6 and 11.1%, respectively, showed CMSP. At 4 h, 35% and at 8 h, 33% of neurons demonstrated CMSP without dextran flooding. At 4 h, 5.5% and at 8 h, 20.9% of tracer-flooded neurons revealed only postinjury dextran uptake, consistent with delayed membrane perturbation, with 55.0 and 35.4%, respectively, showing CMSP. These studies illustrate that DTBI evokes evolving plasmalemmal changes that highlight mechanical and potential secondary events in membrane poration.

Original languageEnglish (US)
Pages (from-to)3130-3140
Number of pages11
JournalJournal of Neuroscience
Volume26
Issue number12
DOIs
StatePublished - Mar 22 2006
Externally publishedYes

Fingerprint

Spectrin
Calpain
Dextrans
Proteolysis
Neurons
Wounds and Injuries
Membranes
Cell Death
Necrosis
Intraventricular Infusions
Diffuse Brain Injury
Traumatic Brain Injury
Confocal Microscopy
Electron Microscopy
Immunohistochemistry

Keywords

  • Calpain
  • Dextrans
  • Diffuse traumatic brain injury
  • Membrane disruption
  • Membrane resealing
  • Neuron

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Mechanoporation induced by diffuse traumatic brain injury : An irreversible or reversible response to injury? / Farkas, Orsolya; Lifshitz, Jonathan; Povlishock, John T.

In: Journal of Neuroscience, Vol. 26, No. 12, 22.03.2006, p. 3130-3140.

Research output: Contribution to journalArticle

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abstract = "Diffuse traumatic brain injury (DTBI) is associated with neuronal plasmalemmal disruption, leading to either necrosis or reactive change without cell death. This study examined whether enduring membrane perturbation consistently occurs, leading to cell death, or if there is the potential for transient perturbation followed by resealing/recovery. We also examined the relationship of these events to calpain-mediated spectrin proteolysis (CMSP). To assess plasmalemmal disruption, rats (n = 21) received intracerebroventricular infusion 2 h before DTBI of a normally excluded 10 kDa fluorophore-labeled dextran. To reveal plasmalemmal resealing or enduring disruption, rats were infused with another labeled dextran 2 h (n = 10) or 6 h (n = 11) after injury. Immunohistochemistry for the 150 kDa spectrin breakdown product evaluated the concomitant role of CMSP. Neocortical neurons were followed with confocal and electron microscopy. After DTBI at 4 and 8 h, 55{\%} of all tracer-flooded neurons contained both dextrans, demonstrating enduring plasmalemmal leakage, with many demonstrating necrosis. At 4 h, 12.0{\%} and at 8 h, 15.7{\%} of the dual tracer-flooded neurons showed CMSP, yet, these demonstrated less advanced cellular change. At 4 h, 39.0{\%} and at 8 h, 24.4{\%} of all tracer-flooded neurons revealed only preinjury dextran uptake, consistent with membrane resealing, whereas 7.6 and 11.1{\%}, respectively, showed CMSP. At 4 h, 35{\%} and at 8 h, 33{\%} of neurons demonstrated CMSP without dextran flooding. At 4 h, 5.5{\%} and at 8 h, 20.9{\%} of tracer-flooded neurons revealed only postinjury dextran uptake, consistent with delayed membrane perturbation, with 55.0 and 35.4{\%}, respectively, showing CMSP. These studies illustrate that DTBI evokes evolving plasmalemmal changes that highlight mechanical and potential secondary events in membrane poration.",
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