Medium weight neurofilament mRNA in goldfish Mauthner axoplasm

Orion D. Weiner, Aaron M. Zorn, Paul A. Krieg, George D. Bittner

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Although axons are generally considered to lack the ability to synthesize proteins, the Mauthner axon (M-axon) of the goldfish has been reported to contain some of the basic components of the translational machinery, such as transfer RNA (tRNA), ribosomal RNA (rRNA), and ribosomes. To determine if the M-axon also contains mRNA, we isolated samples of M-axoplasm free of glial contamination as demonstrated by the absence of glial-specific mRNA and protein. Reverse transcription-polymerase chain reaction (RT-PCR) of M-axoplasmic cDNA in the presence of primers for the goldfish medium-weight neurofilament (NF-M) gene produced a single product of the expected length for RT-PCR amplification of goldfish NF-M mRNA. This mRNA might direct protein synthesis of NF-M within the M-axoplasm.

Original languageEnglish (US)
Pages (from-to)83-86
Number of pages4
JournalNeuroscience Letters
Volume213
Issue number2
DOIs
StatePublished - Aug 2 1996
Externally publishedYes

Keywords

  • Axoplasmic protein synthesis
  • Goldfish Mauthner axon
  • Neurofilament protein
  • mRNA

ASJC Scopus subject areas

  • Neuroscience(all)

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