Melanin concentrating hormone (MCH): Structure-function aspects of its melanocyte stimulating hormone-like (MSH-like) activity

Terry O. Matsunaga, Victor J. Hruby, Michal Lebl, Ana Maria De Lauro Castrucci, Mac E. Hadley

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Melanin concentrating hormone (MCH) is a heptadecapeptide, Asp-Thr-Met-Arg-Cys-Met-Val-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Glu-Val, synthesized in the brain and secreted from the pars nervosa of teleost fish. This hormone stimulates melanosome (melanin granule) aggregation within integumental melanocytes of fishes but, in contrast, stimulates melansome dispersion within tetrapod (frog and lizard) melanocytes. We determined the message sequence of the primary structure of MCH which is responsible for its MSH-like component of activity. Removal of the N-terminal amino acid results in an almost total loss of MSH-like activity. The C-terminal amino acid is also essential for full MSH-like activity since the analogue, MCH(1-16), is about 100 times less active than MCH. Therefore, the entire heptadecapeptide sequence of MCH appears to contribute to the MSH-like activity of MCH. Ring-contracted analogues (e.g., [Ala5,Cys10]MCH) of MCH are almost devoid of any melanosome aggregating (MCH-like) activity but generally possess considerable or as great an MSH-like activity as MCH. Racemization of MCH by heat-alkali treatment drastically reduces the MCH-like activity of MCH, but does not enhance the MSH-like activity of the hormone.

Original languageEnglish (US)
Pages (from-to)773-778
Number of pages6
JournalPeptides
Volume10
Issue number4
DOIs
StatePublished - Jan 1 1989

Keywords

  • Melanin concentrating hormone (MCH)
  • Melanocyte stimulating hormone-like activity
  • Structure-function aspects

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Melanin concentrating hormone (MCH): Structure-function aspects of its melanocyte stimulating hormone-like (MSH-like) activity'. Together they form a unique fingerprint.

  • Cite this