Abstract
Utilizing improved methods, two bacterial artificial chromosome (BAC) libraries were constructed for the multidisease-resistant line of melon MR-1. The HindIII library consists of 177 microtiter plates in a 384-well format, while the EcoRI library consists of 222 microtiter plates. Approximately 95.6% of the HindIII library clones contain nuclear DNA inserts with an average size of 118 kb, providing a coverage of 15.4 genome equivalents. Similarly, 96% of the EcoRI library clones contain nuclear DNA inserts with an average size of 114 kb, providing a coverage of 18.7 genome equivalents. Both libraries were evaluated for contamination with high-copy vector, empty pIndigoBac536 vector, and organellar DNA sequences. High-density filters were screened with two genetic markers FM and AM that co-segregate with Fom-2, a gene conferring resistance to races 0 and 1 of Fusarium wilt. Fourteen and 18 candidate BAC clones were identified for the FM and AM probes, respectively, from the HindIII library, while 34 were identified for the AM probe from filters A, B, and C of the EcoRI library.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 154-162 |
| Number of pages | 9 |
| Journal | Genome |
| Volume | 44 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
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Keywords
- Bacterial artificial chromosome (BAC) library
- Fusarium wilt
- Melon
- pCUGIBAC1
- Resistant gene
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biotechnology
- Genetics
- Genetics(clinical)
Cite this
Melon bacterial artificial chromosome (BAC) library construction using improved methods and identification of clones linked to the locus conferring resistance to melon Fusarium wilt (Fom-2). / Luo, Meizhong; Wang, Yi Hong; Frisch, David; Joobeur, Tarek; Wing, Rod A; Dean, Ralph A.
In: Genome, Vol. 44, No. 2, 2001, p. 154-162.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Melon bacterial artificial chromosome (BAC) library construction using improved methods and identification of clones linked to the locus conferring resistance to melon Fusarium wilt (Fom-2)
AU - Luo, Meizhong
AU - Wang, Yi Hong
AU - Frisch, David
AU - Joobeur, Tarek
AU - Wing, Rod A
AU - Dean, Ralph A.
PY - 2001
Y1 - 2001
N2 - Utilizing improved methods, two bacterial artificial chromosome (BAC) libraries were constructed for the multidisease-resistant line of melon MR-1. The HindIII library consists of 177 microtiter plates in a 384-well format, while the EcoRI library consists of 222 microtiter plates. Approximately 95.6% of the HindIII library clones contain nuclear DNA inserts with an average size of 118 kb, providing a coverage of 15.4 genome equivalents. Similarly, 96% of the EcoRI library clones contain nuclear DNA inserts with an average size of 114 kb, providing a coverage of 18.7 genome equivalents. Both libraries were evaluated for contamination with high-copy vector, empty pIndigoBac536 vector, and organellar DNA sequences. High-density filters were screened with two genetic markers FM and AM that co-segregate with Fom-2, a gene conferring resistance to races 0 and 1 of Fusarium wilt. Fourteen and 18 candidate BAC clones were identified for the FM and AM probes, respectively, from the HindIII library, while 34 were identified for the AM probe from filters A, B, and C of the EcoRI library.
AB - Utilizing improved methods, two bacterial artificial chromosome (BAC) libraries were constructed for the multidisease-resistant line of melon MR-1. The HindIII library consists of 177 microtiter plates in a 384-well format, while the EcoRI library consists of 222 microtiter plates. Approximately 95.6% of the HindIII library clones contain nuclear DNA inserts with an average size of 118 kb, providing a coverage of 15.4 genome equivalents. Similarly, 96% of the EcoRI library clones contain nuclear DNA inserts with an average size of 114 kb, providing a coverage of 18.7 genome equivalents. Both libraries were evaluated for contamination with high-copy vector, empty pIndigoBac536 vector, and organellar DNA sequences. High-density filters were screened with two genetic markers FM and AM that co-segregate with Fom-2, a gene conferring resistance to races 0 and 1 of Fusarium wilt. Fourteen and 18 candidate BAC clones were identified for the FM and AM probes, respectively, from the HindIII library, while 34 were identified for the AM probe from filters A, B, and C of the EcoRI library.
KW - Bacterial artificial chromosome (BAC) library
KW - Fusarium wilt
KW - Melon
KW - pCUGIBAC1
KW - Resistant gene
UR - http://www.scopus.com/inward/record.url?scp=0035056110&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035056110&partnerID=8YFLogxK
U2 - 10.1139/gen-44-2-154
DO - 10.1139/gen-44-2-154
M3 - Article
C2 - 11341724
AN - SCOPUS:0035056110
VL - 44
SP - 154
EP - 162
JO - Genome / National Research Council Canada = Genome / Conseil national de recherches Canada
JF - Genome / National Research Council Canada = Genome / Conseil national de recherches Canada
SN - 0831-2796
IS - 2
ER -