Membrane model of the aortic baroreceptor neuron based upon numerical fits to voltage clamp data

J. H. Schild, Meredith Hay, D. Mendelowitz, M. Priddy, J. W. Clark, M. C. Andresen, D. L. Kunze

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have undertaken to classify and characterize the essential ion channel currents in aortic baroreceptor neurons of neo-natal and juvenile rat. The isolation of these neurons from the milieu of cellular structures within the nodose ganglia was accomplished using a combination of enzymatic dispersion and fluorescence identification (selected juvenile cells). Whole cell current recordings were performed in our laboratory using a cellular patch clamp technique under voltage-and current-clamp conditions. To date, we have identified a single Na+, two Ca2+ and four K+ channels. A nonlinear system of equations capable of accurately describing the dynamics of these ion channels has been configured according to standard Hodgkin-Huxley formalism. Identification of parameters associated with the system state variables was accomplished using a nonlinear least-squares parameter estimation algorithm. This work has provided a unique compendium of ion channel current descriptions which we have assembled into a comprehensive and physiologically consistent Hodgkin-Huxley-type excitable membrane model of the rat aortic baroreceptor neuron. The preliminary configuration of this model along with examples of mathematical fits to our voltage clamp data and action potential waveforms are presented.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsMarry Lou Padgett
PublisherPubl by Society of Photo-Optical Instrumentation Engineers
Pages379-382
Number of pages4
Volume1721
ISBN (Print)1565550072
StatePublished - 1993
Externally publishedYes
EventProceedings of the 3rd Workshop on Neural Networks: Academic/Industrial/NASA/Defense - Alabama, AL, USA
Duration: Feb 10 1992Feb 12 1992

Other

OtherProceedings of the 3rd Workshop on Neural Networks: Academic/Industrial/NASA/Defense
CityAlabama, AL, USA
Period2/10/922/12/92

Fingerprint

baroreceptors
clamps
Clamping devices
neurons
Neurons
membranes
Membranes
Rats
Ions
Electric potential
electric potential
rats
Parameter estimation
Nonlinear systems
nonlinear systems
cells
Fluorescence
isolation
waveforms
recording

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Schild, J. H., Hay, M., Mendelowitz, D., Priddy, M., Clark, J. W., Andresen, M. C., & Kunze, D. L. (1993). Membrane model of the aortic baroreceptor neuron based upon numerical fits to voltage clamp data. In M. L. Padgett (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1721, pp. 379-382). Publ by Society of Photo-Optical Instrumentation Engineers.

Membrane model of the aortic baroreceptor neuron based upon numerical fits to voltage clamp data. / Schild, J. H.; Hay, Meredith; Mendelowitz, D.; Priddy, M.; Clark, J. W.; Andresen, M. C.; Kunze, D. L.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Marry Lou Padgett. Vol. 1721 Publ by Society of Photo-Optical Instrumentation Engineers, 1993. p. 379-382.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Schild, JH, Hay, M, Mendelowitz, D, Priddy, M, Clark, JW, Andresen, MC & Kunze, DL 1993, Membrane model of the aortic baroreceptor neuron based upon numerical fits to voltage clamp data. in ML Padgett (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 1721, Publ by Society of Photo-Optical Instrumentation Engineers, pp. 379-382, Proceedings of the 3rd Workshop on Neural Networks: Academic/Industrial/NASA/Defense, Alabama, AL, USA, 2/10/92.
Schild JH, Hay M, Mendelowitz D, Priddy M, Clark JW, Andresen MC et al. Membrane model of the aortic baroreceptor neuron based upon numerical fits to voltage clamp data. In Padgett ML, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1721. Publ by Society of Photo-Optical Instrumentation Engineers. 1993. p. 379-382
Schild, J. H. ; Hay, Meredith ; Mendelowitz, D. ; Priddy, M. ; Clark, J. W. ; Andresen, M. C. ; Kunze, D. L. / Membrane model of the aortic baroreceptor neuron based upon numerical fits to voltage clamp data. Proceedings of SPIE - The International Society for Optical Engineering. editor / Marry Lou Padgett. Vol. 1721 Publ by Society of Photo-Optical Instrumentation Engineers, 1993. pp. 379-382
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