Membrane potential modulates plasma membrane phospholipid dynamics and K-Ras signaling

Yong Zhou, Ching On Wong, Kwang Jin Cho, Dharini Van Der Hoeven, Hong Liang, Dhananiay P. Thakur, Jialie Luo, Milos Babic, Konrad E Zinsmaier, Michael X. Zhu, Hongzhen Hu, Kartik Venkatachalam, John F. Hancock

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Plasma membrane depolarization can trigger cell proliferation, but how membrane potential influences mitogenic signaling is uncertain. Here, we show that plasma membrane depolarization induces nanoscale reorganization of phosphatidylserine and phosphatidylinositol 4,5-bisphosphate but not other anionic phospholipids. K-Ras, which is targeted to the plasma membrane by electrostatic interactions with phosphatidylserine, in turn undergoes enhanced nanoclustering. Depolarization-induced changes in phosphatidylserine and K-Ras plasma membrane organization occur in fibroblasts, excitable neuroblastoma cells, and Drosophila neurons in vivo and robustly amplify K-Ras-dependent mitogen-activated protein kinase (MAPK) signaling. Conversely, plasma membrane repolarization disrupts K-Ras nanoclustering and inhibits MAPK signaling. By responding to voltage-induced changes in phosphatidylserine spatiotemporal dynamics, K-Ras nanoclusters set up the plasma membrane as a biological field-effect transistor, allowing membrane potential to control the gain in mitogenic signaling circuits.

Original languageEnglish (US)
Pages (from-to)873-876
Number of pages4
JournalScience
Volume349
Issue number6250
DOIs
StatePublished - Aug 21 2015

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Membrane Potentials
Phospholipids
Cell Membrane
Phosphatidylserines
Mitogen-Activated Protein Kinases
Phosphatidylinositols
Static Electricity
Neuroblastoma
Drosophila
Fibroblasts
Cell Proliferation
Neurons

ASJC Scopus subject areas

  • General

Cite this

Zhou, Y., Wong, C. O., Cho, K. J., Van Der Hoeven, D., Liang, H., Thakur, D. P., ... Hancock, J. F. (2015). Membrane potential modulates plasma membrane phospholipid dynamics and K-Ras signaling. Science, 349(6250), 873-876. https://doi.org/10.1126/science.aaa5619

Membrane potential modulates plasma membrane phospholipid dynamics and K-Ras signaling. / Zhou, Yong; Wong, Ching On; Cho, Kwang Jin; Van Der Hoeven, Dharini; Liang, Hong; Thakur, Dhananiay P.; Luo, Jialie; Babic, Milos; Zinsmaier, Konrad E; Zhu, Michael X.; Hu, Hongzhen; Venkatachalam, Kartik; Hancock, John F.

In: Science, Vol. 349, No. 6250, 21.08.2015, p. 873-876.

Research output: Contribution to journalArticle

Zhou, Y, Wong, CO, Cho, KJ, Van Der Hoeven, D, Liang, H, Thakur, DP, Luo, J, Babic, M, Zinsmaier, KE, Zhu, MX, Hu, H, Venkatachalam, K & Hancock, JF 2015, 'Membrane potential modulates plasma membrane phospholipid dynamics and K-Ras signaling', Science, vol. 349, no. 6250, pp. 873-876. https://doi.org/10.1126/science.aaa5619
Zhou Y, Wong CO, Cho KJ, Van Der Hoeven D, Liang H, Thakur DP et al. Membrane potential modulates plasma membrane phospholipid dynamics and K-Ras signaling. Science. 2015 Aug 21;349(6250):873-876. https://doi.org/10.1126/science.aaa5619
Zhou, Yong ; Wong, Ching On ; Cho, Kwang Jin ; Van Der Hoeven, Dharini ; Liang, Hong ; Thakur, Dhananiay P. ; Luo, Jialie ; Babic, Milos ; Zinsmaier, Konrad E ; Zhu, Michael X. ; Hu, Hongzhen ; Venkatachalam, Kartik ; Hancock, John F. / Membrane potential modulates plasma membrane phospholipid dynamics and K-Ras signaling. In: Science. 2015 ; Vol. 349, No. 6250. pp. 873-876.
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