Membrane-tethered MUC1 mucin counter-regulates the phagocytic activity of macrophages

Kosuke Kato, Reina Uchino, Erik P. Lillehoj, Kenneth S Knox, Yong Lin, K. Chul Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

MUC1 (MUC in human; Muc in animals) is a transmembrane mucin glycoprotein expressed in mucosal epithelial cells and hematopoietic cells. MUC1 is involved in the resolution of inflammation during airway Pseudomonas aeruginosa (Pa) infection by suppressing Toll-like receptor signaling in airway epithelial cells. Although alveolar macrophages are recognized as critical mediators of cell-mediated immunity against microorganisms inhaled into the airways, the role of MUC1 in regulating their response is unknown. The aims of this study were to determine whether macrophages express MUC1, and, if so, whetherMUC1 expression might be associated with macrophage M0/M1/M2 differentiation or phagocytic activity. Human and mouse MUC1/Muc1 expression was drastically up-regulated in classically activated (M1) macrophages compared with nonactivated (M0) and alternatively activated (M2) macrophages. M1 polarization and Pa stimulation each increased MUC1 ectodomain shedding from the macrophage surface in a TNF-a-converting enzyme-dependent manner. MUC1/Muc1 deficiency in M0 macrophages increased adhesion and phagocytosis of Pa and Escherichia coli compared with MUC1/Muc1-expressing cells, and attenuation of phagocytosis by MUC1 was augmented after polarization into M1 macrophages compared with M0 macrophages. Finally, MUC1/Muc1 deficiency in macrophages increased reactive oxygen species production and TNF-a release in response to Pa compared with MUC1/Muc1-sufficient cells. These results indicate that MUC1/Muc1 expression by macrophages is predominantly in the M1 subtype, and that MUC1/Muc1 expression in these cells decreases their phagocytic activity in an antiinflammatory manner.

Original languageEnglish (US)
Pages (from-to)515-523
Number of pages9
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume54
Issue number4
DOIs
StatePublished - Apr 1 2016

Fingerprint

Macrophages
Mucins
Membranes
Pseudomonas aeruginosa
Epithelial Cells
Polarization
Cytophagocytosis
Pseudomonas Infections
Toll-Like Receptors
Alveolar Macrophages
Phagocytosis
Cellular Immunity
Microorganisms
Escherichia coli
Reactive Oxygen Species
Glycoproteins
Animals
Anti-Inflammatory Agents
Adhesion
Inflammation

Keywords

  • Inflammation
  • Macrophage
  • MUC1 mucin
  • Phagocytosis
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Membrane-tethered MUC1 mucin counter-regulates the phagocytic activity of macrophages. / Kato, Kosuke; Uchino, Reina; Lillehoj, Erik P.; Knox, Kenneth S; Lin, Yong; Kim, K. Chul.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 54, No. 4, 01.04.2016, p. 515-523.

Research output: Contribution to journalArticle

Kato, Kosuke ; Uchino, Reina ; Lillehoj, Erik P. ; Knox, Kenneth S ; Lin, Yong ; Kim, K. Chul. / Membrane-tethered MUC1 mucin counter-regulates the phagocytic activity of macrophages. In: American Journal of Respiratory Cell and Molecular Biology. 2016 ; Vol. 54, No. 4. pp. 515-523.
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