Memory consolidation and gene expression in Periplaneta americana

Marianna Pintér, David D. Lent, Nicholas J Strausfeld

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A unique behavioral paradigm has been developed for Periplaneta americana that assesses the timing and success of memory consolidation leading to long-term memory of visual-olfactory associations. The brains of trained and control animals, removed at the critical consolidation period, were screened by two-directional suppression subtractive hybridization. Screens identified neurobiologically relevant as well as novel genes that are differentially expressed at the consolidation phase of memory. The differential expression of six transcripts was confirmed with real-time RT-PCR experiments. There are mitochondrial DNA encoded transcripts among the up-regulated ones (COX, ATPase6). One of the confirmed down-regulated transcripts is RNA polymerase II largest subunit. The mitochondrial genes are of particular interest because mitochondria represent autonomous DNA at synapses. These transcripts will be used as one of several tools in the identification of neuronal circuits, such as in the mushroom bodies, that are implicated in memory consolidation.

Original languageEnglish (US)
Pages (from-to)30-38
Number of pages9
JournalLearning and Memory
Volume12
Issue number1
DOIs
StatePublished - Jan 2005

Fingerprint

Periplaneta
Gene Expression
Mushroom Bodies
Mitochondrial Genes
Long-Term Memory
Mitochondrial DNA
Synapses
Real-Time Polymerase Chain Reaction
Mitochondria
DNA
Brain
Genes
Memory Consolidation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Memory consolidation and gene expression in Periplaneta americana. / Pintér, Marianna; Lent, David D.; Strausfeld, Nicholas J.

In: Learning and Memory, Vol. 12, No. 1, 01.2005, p. 30-38.

Research output: Contribution to journalArticle

Pintér, Marianna ; Lent, David D. ; Strausfeld, Nicholas J. / Memory consolidation and gene expression in Periplaneta americana. In: Learning and Memory. 2005 ; Vol. 12, No. 1. pp. 30-38.
@article{2d4ac0de9c4640fcb124a9baf5b09c39,
title = "Memory consolidation and gene expression in Periplaneta americana",
abstract = "A unique behavioral paradigm has been developed for Periplaneta americana that assesses the timing and success of memory consolidation leading to long-term memory of visual-olfactory associations. The brains of trained and control animals, removed at the critical consolidation period, were screened by two-directional suppression subtractive hybridization. Screens identified neurobiologically relevant as well as novel genes that are differentially expressed at the consolidation phase of memory. The differential expression of six transcripts was confirmed with real-time RT-PCR experiments. There are mitochondrial DNA encoded transcripts among the up-regulated ones (COX, ATPase6). One of the confirmed down-regulated transcripts is RNA polymerase II largest subunit. The mitochondrial genes are of particular interest because mitochondria represent autonomous DNA at synapses. These transcripts will be used as one of several tools in the identification of neuronal circuits, such as in the mushroom bodies, that are implicated in memory consolidation.",
author = "Marianna Pint{\'e}r and Lent, {David D.} and Strausfeld, {Nicholas J}",
year = "2005",
month = "1",
doi = "10.1101/lm.87905",
language = "English (US)",
volume = "12",
pages = "30--38",
journal = "Learning and Memory",
issn = "1072-0502",
publisher = "Cold Spring Harbor Laboratory Press",
number = "1",

}

TY - JOUR

T1 - Memory consolidation and gene expression in Periplaneta americana

AU - Pintér, Marianna

AU - Lent, David D.

AU - Strausfeld, Nicholas J

PY - 2005/1

Y1 - 2005/1

N2 - A unique behavioral paradigm has been developed for Periplaneta americana that assesses the timing and success of memory consolidation leading to long-term memory of visual-olfactory associations. The brains of trained and control animals, removed at the critical consolidation period, were screened by two-directional suppression subtractive hybridization. Screens identified neurobiologically relevant as well as novel genes that are differentially expressed at the consolidation phase of memory. The differential expression of six transcripts was confirmed with real-time RT-PCR experiments. There are mitochondrial DNA encoded transcripts among the up-regulated ones (COX, ATPase6). One of the confirmed down-regulated transcripts is RNA polymerase II largest subunit. The mitochondrial genes are of particular interest because mitochondria represent autonomous DNA at synapses. These transcripts will be used as one of several tools in the identification of neuronal circuits, such as in the mushroom bodies, that are implicated in memory consolidation.

AB - A unique behavioral paradigm has been developed for Periplaneta americana that assesses the timing and success of memory consolidation leading to long-term memory of visual-olfactory associations. The brains of trained and control animals, removed at the critical consolidation period, were screened by two-directional suppression subtractive hybridization. Screens identified neurobiologically relevant as well as novel genes that are differentially expressed at the consolidation phase of memory. The differential expression of six transcripts was confirmed with real-time RT-PCR experiments. There are mitochondrial DNA encoded transcripts among the up-regulated ones (COX, ATPase6). One of the confirmed down-regulated transcripts is RNA polymerase II largest subunit. The mitochondrial genes are of particular interest because mitochondria represent autonomous DNA at synapses. These transcripts will be used as one of several tools in the identification of neuronal circuits, such as in the mushroom bodies, that are implicated in memory consolidation.

UR - http://www.scopus.com/inward/record.url?scp=13444302364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13444302364&partnerID=8YFLogxK

U2 - 10.1101/lm.87905

DO - 10.1101/lm.87905

M3 - Article

VL - 12

SP - 30

EP - 38

JO - Learning and Memory

JF - Learning and Memory

SN - 1072-0502

IS - 1

ER -