Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy

Janine Mauzeroll, Allen J. Bard, Omeed Owhadian, Terrence J. Monks

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

The cytotoxicity of menadione on hepatocytes was studied by using the substrate generation/tip collection mode of scanning electrochemical microscopy by exposing the cells to menadione and detecting the menadione-S-glutathione conjugate (thiodione) that is formed during the cellular detoxication process and is exported from the cell by an ATP-dependent pump. This efflux was electrochemically detected and allowed scanning electrochemical microscopy monitoring and imaging of single cells and groups of highly confluent live cells. Based on a constant flux model, ≈6 × 106 molecules of thiodione per cell per second are exported from monolayer cultures of Hep G2 cells.

Original languageEnglish (US)
Pages (from-to)17582-17587
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number51
DOIs
StatePublished - Dec 21 2004

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Keywords

  • Electrochemistry
  • Glutathione
  • Oxidative stress
  • SECM
  • Ultramicroelectrode

ASJC Scopus subject areas

  • General

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