MeroGel hyaluronic acid sinonasal implants: Osteogenic implications

Abraham Jacob, Brian T. Faddis, Richard A. Chole

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Objectives/Hypothesis: The study sought to evaluate the effects of MeroGel (Medtronic Xomed Surgical Products, Jacksonville, FL) hyaluronic acid sinonasal implants by using a mouse model. We expected that this material would dissolve, elicit minimal inflammation, and leave intact mucosa. However, pilot data showed that MeroGel may be osteogenic. The present work focuses on the description and quantification of these bony changes. Study Design: Animal experiment. Methods: The effects of MeroGel were evaluated using sinonasal and calvarial implants in C57BL/6 mice. The calvarial experiments employed both light microscopy (bone histomorphometry) and fluorochrome labeling (mineral apposition). Each implant study was subdivided into normal control animals, sham-operated control animals, a foreign body control, and MeroGel implants. MeroGel was also placed within subcutaneous pockets remote from bone. All mice were killed 1 month after surgery. Both percentage of bone area within the sinonasal cavity and total calvarial bone area were quantified and compared. Fluorochrome images and the subcutaneous implants were examined qualitatively. Results: MeroGel placement engendered new bone formation within the nasal lumen of mice. Compared with normal control animals, sham-operated control animals, and a foreign body control, this increase was statistically significant. The calvaria from MeroGel-implanted mice were thickened as compared with normal animals, sham-operated control animals, and a foreign body control. Tetracycline labeling demonstrated mineral apposition along the dorsal and ventral surfaces of the calvaria and within enlarged marrow spaces. MeroGel placed subcutaneously did not engender bone formation. Conclusions: MeroGel, a unique esterified form of hyaluronic acid, induced bone formation within the sinonasal cavity and on the calvaria of mice implanted with it. Our experiments suggest that, when placed adjacent to traumatized, remodeling bone, MeroGel may have osteogenic potential.

Original languageEnglish (US)
Pages (from-to)37-42
Number of pages6
JournalLaryngoscope
Volume112
Issue number1
StatePublished - 2002
Externally publishedYes

Fingerprint

Hyaluronic Acid
Foreign Bodies
Osteogenesis
Skull
Bone and Bones
Fluorescent Dyes
Minerals
Bone Remodeling
Tetracycline
Inbred C57BL Mouse
Nose
Microscopy
Mucous Membrane
Bone Marrow
Inflammation
Light

Keywords

  • Bone formation
  • Chronic sinusitis
  • Hyaluronic acid
  • MeroGel
  • Mouse

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Jacob, A., Faddis, B. T., & Chole, R. A. (2002). MeroGel hyaluronic acid sinonasal implants: Osteogenic implications. Laryngoscope, 112(1), 37-42.

MeroGel hyaluronic acid sinonasal implants : Osteogenic implications. / Jacob, Abraham; Faddis, Brian T.; Chole, Richard A.

In: Laryngoscope, Vol. 112, No. 1, 2002, p. 37-42.

Research output: Contribution to journalArticle

Jacob, A, Faddis, BT & Chole, RA 2002, 'MeroGel hyaluronic acid sinonasal implants: Osteogenic implications', Laryngoscope, vol. 112, no. 1, pp. 37-42.
Jacob, Abraham ; Faddis, Brian T. ; Chole, Richard A. / MeroGel hyaluronic acid sinonasal implants : Osteogenic implications. In: Laryngoscope. 2002 ; Vol. 112, No. 1. pp. 37-42.
@article{e2fc70b0085646de86e4a99a6005da19,
title = "MeroGel hyaluronic acid sinonasal implants: Osteogenic implications",
abstract = "Objectives/Hypothesis: The study sought to evaluate the effects of MeroGel (Medtronic Xomed Surgical Products, Jacksonville, FL) hyaluronic acid sinonasal implants by using a mouse model. We expected that this material would dissolve, elicit minimal inflammation, and leave intact mucosa. However, pilot data showed that MeroGel may be osteogenic. The present work focuses on the description and quantification of these bony changes. Study Design: Animal experiment. Methods: The effects of MeroGel were evaluated using sinonasal and calvarial implants in C57BL/6 mice. The calvarial experiments employed both light microscopy (bone histomorphometry) and fluorochrome labeling (mineral apposition). Each implant study was subdivided into normal control animals, sham-operated control animals, a foreign body control, and MeroGel implants. MeroGel was also placed within subcutaneous pockets remote from bone. All mice were killed 1 month after surgery. Both percentage of bone area within the sinonasal cavity and total calvarial bone area were quantified and compared. Fluorochrome images and the subcutaneous implants were examined qualitatively. Results: MeroGel placement engendered new bone formation within the nasal lumen of mice. Compared with normal control animals, sham-operated control animals, and a foreign body control, this increase was statistically significant. The calvaria from MeroGel-implanted mice were thickened as compared with normal animals, sham-operated control animals, and a foreign body control. Tetracycline labeling demonstrated mineral apposition along the dorsal and ventral surfaces of the calvaria and within enlarged marrow spaces. MeroGel placed subcutaneously did not engender bone formation. Conclusions: MeroGel, a unique esterified form of hyaluronic acid, induced bone formation within the sinonasal cavity and on the calvaria of mice implanted with it. Our experiments suggest that, when placed adjacent to traumatized, remodeling bone, MeroGel may have osteogenic potential.",
keywords = "Bone formation, Chronic sinusitis, Hyaluronic acid, MeroGel, Mouse",
author = "Abraham Jacob and Faddis, {Brian T.} and Chole, {Richard A.}",
year = "2002",
language = "English (US)",
volume = "112",
pages = "37--42",
journal = "Laryngoscope",
issn = "0023-852X",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - MeroGel hyaluronic acid sinonasal implants

T2 - Osteogenic implications

AU - Jacob, Abraham

AU - Faddis, Brian T.

AU - Chole, Richard A.

PY - 2002

Y1 - 2002

N2 - Objectives/Hypothesis: The study sought to evaluate the effects of MeroGel (Medtronic Xomed Surgical Products, Jacksonville, FL) hyaluronic acid sinonasal implants by using a mouse model. We expected that this material would dissolve, elicit minimal inflammation, and leave intact mucosa. However, pilot data showed that MeroGel may be osteogenic. The present work focuses on the description and quantification of these bony changes. Study Design: Animal experiment. Methods: The effects of MeroGel were evaluated using sinonasal and calvarial implants in C57BL/6 mice. The calvarial experiments employed both light microscopy (bone histomorphometry) and fluorochrome labeling (mineral apposition). Each implant study was subdivided into normal control animals, sham-operated control animals, a foreign body control, and MeroGel implants. MeroGel was also placed within subcutaneous pockets remote from bone. All mice were killed 1 month after surgery. Both percentage of bone area within the sinonasal cavity and total calvarial bone area were quantified and compared. Fluorochrome images and the subcutaneous implants were examined qualitatively. Results: MeroGel placement engendered new bone formation within the nasal lumen of mice. Compared with normal control animals, sham-operated control animals, and a foreign body control, this increase was statistically significant. The calvaria from MeroGel-implanted mice were thickened as compared with normal animals, sham-operated control animals, and a foreign body control. Tetracycline labeling demonstrated mineral apposition along the dorsal and ventral surfaces of the calvaria and within enlarged marrow spaces. MeroGel placed subcutaneously did not engender bone formation. Conclusions: MeroGel, a unique esterified form of hyaluronic acid, induced bone formation within the sinonasal cavity and on the calvaria of mice implanted with it. Our experiments suggest that, when placed adjacent to traumatized, remodeling bone, MeroGel may have osteogenic potential.

AB - Objectives/Hypothesis: The study sought to evaluate the effects of MeroGel (Medtronic Xomed Surgical Products, Jacksonville, FL) hyaluronic acid sinonasal implants by using a mouse model. We expected that this material would dissolve, elicit minimal inflammation, and leave intact mucosa. However, pilot data showed that MeroGel may be osteogenic. The present work focuses on the description and quantification of these bony changes. Study Design: Animal experiment. Methods: The effects of MeroGel were evaluated using sinonasal and calvarial implants in C57BL/6 mice. The calvarial experiments employed both light microscopy (bone histomorphometry) and fluorochrome labeling (mineral apposition). Each implant study was subdivided into normal control animals, sham-operated control animals, a foreign body control, and MeroGel implants. MeroGel was also placed within subcutaneous pockets remote from bone. All mice were killed 1 month after surgery. Both percentage of bone area within the sinonasal cavity and total calvarial bone area were quantified and compared. Fluorochrome images and the subcutaneous implants were examined qualitatively. Results: MeroGel placement engendered new bone formation within the nasal lumen of mice. Compared with normal control animals, sham-operated control animals, and a foreign body control, this increase was statistically significant. The calvaria from MeroGel-implanted mice were thickened as compared with normal animals, sham-operated control animals, and a foreign body control. Tetracycline labeling demonstrated mineral apposition along the dorsal and ventral surfaces of the calvaria and within enlarged marrow spaces. MeroGel placed subcutaneously did not engender bone formation. Conclusions: MeroGel, a unique esterified form of hyaluronic acid, induced bone formation within the sinonasal cavity and on the calvaria of mice implanted with it. Our experiments suggest that, when placed adjacent to traumatized, remodeling bone, MeroGel may have osteogenic potential.

KW - Bone formation

KW - Chronic sinusitis

KW - Hyaluronic acid

KW - MeroGel

KW - Mouse

UR - http://www.scopus.com/inward/record.url?scp=0036138660&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036138660&partnerID=8YFLogxK

M3 - Article

C2 - 11802036

AN - SCOPUS:0036138660

VL - 112

SP - 37

EP - 42

JO - Laryngoscope

JF - Laryngoscope

SN - 0023-852X

IS - 1

ER -