Messenger RNA 5′ NAD+ Capping Is a Dynamic Regulatory Epitranscriptome Mark That Is Required for Proper Response to Abscisic Acid in Arabidopsis

Xiang Yu, Matthew R. Willmann, Lee E. Vandivier, Sophie Trefely, Marianne C. Kramer, Jeffrey Shapiro, Rong Guo, Eric Lyons, Nathaniel W. Snyder, Brian D. Gregory

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Although eukaryotic messenger RNAs (mRNAs) normally possess a 5′ end N7-methyl guanosine (m7G) cap, a non-canonical 5′ nicotinamide adenine dinucleotide (NAD+) cap can tag certain transcripts for degradation mediated by the NAD+ decapping enzyme DXO1. Despite this importance, whether NAD+ capping dynamically responds to specific stimuli to regulate eukaryotic transcriptomes remains unknown. Here, we reveal a link between NAD+ capping and tissue- and hormone response-specific mRNA stability. In the absence of DXO1 function, transcripts displaying a high proportion of NAD+ capping are instead processed into RNA-dependent RNA polymerase 6-dependent small RNAs, resulting in their continued turnover likely to free the NAD+ molecules. Additionally, the NAD+-capped transcriptome is significantly remodeled in response to the essential plant hormone abscisic acid in a mechanism that is primarily independent of DXO1. Overall, our findings reveal a previously uncharacterized and essential role of NAD+ capping in dynamically regulating transcript stability during specific physiological responses.

Original languageEnglish (US)
Pages (from-to)125-140.e6
JournalDevelopmental Cell
Volume56
Issue number1
DOIs
StatePublished - Jan 11 2021

Keywords

  • ABA
  • NAD capping
  • RNA degradation
  • RNA modification
  • RNA stability
  • abscisic acid
  • epitranscriptome
  • post-transcriptional regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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