Metabolic resistance to organophosphate insecticides in natural populations of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in Egypt and molecular identification of mitotypes

El Sayed H. Shaurub, Jorge R. Paredes-Montero, Judith K. Brown, Haggag S. Zein, Amr A. Mohamed

Research output: Contribution to journalArticlepeer-review

Abstract

The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) cryptic species has become an important pest of cotton and vegetable crops in Egypt. In this study, resistance to three organophosphate insecticides (OPs), chlorpyrifos-methyl, profenofos and pirimiphos-methyl, and detoxification enzymatic activities of acetylcholinesterase, carboxylesterase, and monooxygenase were evaluated to establish baseline resistance levels for B. tabaci infesting cotton in seven Egyptian governorates, compared to a susceptible laboratory reference B. tabaci colony. Resistance to OPs ranged from low-to-moderate or low-to-high, while detoxification enzymatic activities were predominantly governorate-dependent. Phylogenetic analysis of a fragment of the mitochondria cytochrome oxidase I gene (756 bp) identified field-collected B. tabaci as the B mitotype. The results provide baseline OP resistance levels for B. tabaci that will aid in interpreting future monitoring outcomes and enable detection of insecticide resistance fluxes.

Original languageEnglish (US)
JournalPhytoparasitica
DOIs
StateAccepted/In press - 2020

Keywords

  • B mitotype
  • Cotton
  • Phylogeny
  • Resistance monitoring
  • Virus vector

ASJC Scopus subject areas

  • Plant Science
  • Insect Science

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