Metabolite profile analysis reveals functional effects of 28-day vitamin b-6 restriction on one-carbon metabolism and tryptophan catabolic pathways in healthymen andwomen

Vanessa R. da Silva, Luisa Rios-Avila, Yvonne Lamers, Maria A. Ralat, Øivind Midttun, Eoin P. Quinlivan, Timothy J. Garrett, Bonnie Coats, Meena N. Shankar, Susan S. Percival, Yueh Yun Chi, Keith E. Muller, Per Magne Ueland, Peter W. Stacpoole, Jesse F. Gregory

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30 Scopus citations

Abstract

Suboptimal vitamin B-6 status, as reflected by low plasma pyridoxal 5#-phosphate (PLP) concentration, is associated with increased risk of vascular disease. PLP plays many roles, including in one-carbon metabolism for the acquisition and transfer of carbon units and in the transsulfuration pathway. PLP also serves as a coenzyme in the catabolism of tryptophan. We hypothesize that the pattern of these metabolites can provide information reflecting the functional impact of marginal vitamin B-6 deficiency. We report here the concentration of major constituents of one-carbon metabolic processes and the tryptophan catabolic pathway in plasma from 23 healthy men and women before and after a 28-d controlled dietary vitamin B-6 restriction (<0.35 mg/d). liquid chromatography-tandem mass spectrometry analysis of the compounds relevant to one-carbon metabolism showed that vitamin B-6 restriction yielded increased cystathionine (53% pre-and 76% postprandial; P < 0.0001) and serine (12% preprandial; P < 0.05), and lower creatine (40% pre-and postprandial; P < 0.0001), creatinine (9% postprandial; P < 0.05), and dimethylglycine (16% postprandial; P < 0.05) relative to the vitamin B-6-adequate state. In the tryptophan pathway, vitamin B-6 restriction yielded lower kynurenic acid (22% pre-and 20% postprandial; P < 0.01) and higher 3-hydroxykynurenine (39% pre-and 34%postprandial; P < 0.01). Multivariate ANOVA analysis showed a significant global effect of vitamin B-6 restriction and multilevel partial least squares-discriminant analysis supported this conclusion. Thus, plasma concentrations of creatine, cystathionine, kynurenic acid, and 3-hydroxykynurenine jointly reveal effects of vitamin B-6 restriction on the profiles of one-carbon and tryptophan metabolites and serve as biomarkers of functional effects of marginal vitamin B-6 deficiency. J. Nutr. 143: 1719-1727, 2013.

Original languageEnglish (US)
Pages (from-to)1719-1727
Number of pages9
JournalJournal of Nutrition
Volume143
Issue number11
DOIs
StatePublished - Nov 2013
Externally publishedYes

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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    da Silva, V. R., Rios-Avila, L., Lamers, Y., Ralat, M. A., Midttun, Ø., Quinlivan, E. P., Garrett, T. J., Coats, B., Shankar, M. N., Percival, S. S., Chi, Y. Y., Muller, K. E., Ueland, P. M., Stacpoole, P. W., & Gregory, J. F. (2013). Metabolite profile analysis reveals functional effects of 28-day vitamin b-6 restriction on one-carbon metabolism and tryptophan catabolic pathways in healthymen andwomen. Journal of Nutrition, 143(11), 1719-1727. https://doi.org/10.3945/jn.113.180588