Metabotropic glutamate receptor agonists regulate phosphorylation on synapsin I in visceral sensory neurons

K. Lindsley, E. Price, E. M. Hasser, Meredith Hay

Research output: Contribution to journalArticle

Abstract

Metabotropic glutamate receptors (mGluRs) have been hypothesized to serve as presynaptic autoreceptors which may regulate neurotransmitter release in glutamatergic neurons. The mechanisms involved in this inhibition of transmitter release are unknown. Synapsin I is a nerve terminal associated protein which, when phosphorylated, is known to potentiate the release of neurotransmitter. Recent studies in our laboratory have identified the presence of synapsin I protein in the terminal regions of aortic baroreceptors maintained in culture. This study tested the hypothesis that mGluRs may modulate neurotransmitter release in visceral sensory neurons by regulating the phosphorylation of snyapsin I. Rat nodose-ganglia dissociated cultures were maintained in serum-free media for 2 weeks. Cells were incubated in[32P]orthophosphate and depolarized by addition of 60 mM KCl. Depolarization of the nodose neurons with 60 mM KCl increased phosphorylation of synapsin I relative to control by nearly 40%. To test the effects of activation of metabotropic receptors on this response, a cocktail mGluR ligands designed to activate multiple subtypes of mGluRs was used and included 1.0 mM t-ACPD and 1.0 mM L-AP4. mGluR ligands were added 15 min. prior to depolarization. Addition of the mGluR ligands markedly reduced the observed phosphorylation (or increased the dephosphorylation) in KCl depolarized cells. These results suggest the mGluR presynaptic regulation of neurotransmitter release may involve the modulation of synapsin I phosphorylation.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

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Synapsins
Excitatory Amino Acid Agonists
Phosphorylation
Metabotropic Glutamate Receptors
sensory neurons
Sensory Receptor Cells
neurotransmitters
agonists
Neurons
Neurotransmitter Agents
phosphorylation
Depolarization
Ligands
neurons
Nodose Ganglion
Autoreceptors
Pressoreceptors
dephosphorylation
orthophosphates
Serum-Free Culture Media

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Metabotropic glutamate receptor agonists regulate phosphorylation on synapsin I in visceral sensory neurons. / Lindsley, K.; Price, E.; Hasser, E. M.; Hay, Meredith.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

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