Methacrylate Polymer Scaffolding Enhances the Stability of Suspended Lipid Bilayers for Ion Channel Recordings and Biosensor Development

Leonard K. Bright, Christopher A. Baker, Robert Bränström, Steven S Saavedra, Craig A Aspinwall

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Black lipid membranes (BLMs) provide a synthetic environment that facilitates measurement of ion channel activity in diverse analytical platforms. The limited electrical, mechanical and temporal stabilities of BLMs pose a significant challenge to development of highly stable measurement platforms. Here, ethylene glycol dimethacrylate (EGDMA) and butyl methacrylate (BMA) were partitioned into BLMs and photopolymerized to create a cross-linked polymer scaffold in the bilayer lamella that dramatically improved BLM stability. The commercially available methacrylate monomers provide a simple, low cost, and broadly accessible approach for preparing highly stabilized BLMs useful for ion channel-functionalized analytical platforms. When prepared on silane-modified glass microapertures, the resulting polymer scaffold-stabilized (PSS)-BLMs exhibited significantly improved lifetimes of 23 ± 9 to 40 ± 14 h and >10-fold increase in mechanical stability, with breakdown potentials >2000 mV attainable, depending on surface modification and polymer cross-link density. Additionally, the polymer scaffold exerted minimal perturbation to membrane electrical integrity as indicated by mean conductance measurements. When gramicidin A and α-hemolysin were reconstituted into PSS-BLMs, the ion channels retained function comparable to conventional BLMs. This approach is a key advance in the formation of stabilized BLMs and should be amenable to a wide range of receptor and ion channel functionalized platforms.

Original languageEnglish (US)
Pages (from-to)955-963
Number of pages9
JournalACS Biomaterials Science and Engineering
Volume1
Issue number10
DOIs
StatePublished - Oct 12 2015

Fingerprint

Lipid bilayers
Methacrylates
Membrane Lipids
Ion Channels
Biosensors
Polymers
Ions
Scaffolds
Gramicidin
Silanes
Hemolysin Proteins
Mechanical stability
Ethylene glycol
Surface treatment
Monomers
Membranes
Glass

Keywords

  • black lipid membrane
  • gramicidin
  • ion channel
  • lipid bilayer
  • methacrylate polymer
  • polymer scaffold
  • α-hemolysin

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Methacrylate Polymer Scaffolding Enhances the Stability of Suspended Lipid Bilayers for Ion Channel Recordings and Biosensor Development. / Bright, Leonard K.; Baker, Christopher A.; Bränström, Robert; Saavedra, Steven S; Aspinwall, Craig A.

In: ACS Biomaterials Science and Engineering, Vol. 1, No. 10, 12.10.2015, p. 955-963.

Research output: Contribution to journalArticle

@article{531a2f5cce614da19a2b29b97f31c9b8,
title = "Methacrylate Polymer Scaffolding Enhances the Stability of Suspended Lipid Bilayers for Ion Channel Recordings and Biosensor Development",
abstract = "Black lipid membranes (BLMs) provide a synthetic environment that facilitates measurement of ion channel activity in diverse analytical platforms. The limited electrical, mechanical and temporal stabilities of BLMs pose a significant challenge to development of highly stable measurement platforms. Here, ethylene glycol dimethacrylate (EGDMA) and butyl methacrylate (BMA) were partitioned into BLMs and photopolymerized to create a cross-linked polymer scaffold in the bilayer lamella that dramatically improved BLM stability. The commercially available methacrylate monomers provide a simple, low cost, and broadly accessible approach for preparing highly stabilized BLMs useful for ion channel-functionalized analytical platforms. When prepared on silane-modified glass microapertures, the resulting polymer scaffold-stabilized (PSS)-BLMs exhibited significantly improved lifetimes of 23 ± 9 to 40 ± 14 h and >10-fold increase in mechanical stability, with breakdown potentials >2000 mV attainable, depending on surface modification and polymer cross-link density. Additionally, the polymer scaffold exerted minimal perturbation to membrane electrical integrity as indicated by mean conductance measurements. When gramicidin A and α-hemolysin were reconstituted into PSS-BLMs, the ion channels retained function comparable to conventional BLMs. This approach is a key advance in the formation of stabilized BLMs and should be amenable to a wide range of receptor and ion channel functionalized platforms.",
keywords = "black lipid membrane, gramicidin, ion channel, lipid bilayer, methacrylate polymer, polymer scaffold, α-hemolysin",
author = "Bright, {Leonard K.} and Baker, {Christopher A.} and Robert Br{\"a}nstr{\"o}m and Saavedra, {Steven S} and Aspinwall, {Craig A}",
year = "2015",
month = "10",
day = "12",
doi = "10.1021/acsbiomaterials.5b00205",
language = "English (US)",
volume = "1",
pages = "955--963",
journal = "ACS Biomaterials Science and Engineering",
issn = "2373-9878",
publisher = "American Chemical Society",
number = "10",

}

TY - JOUR

T1 - Methacrylate Polymer Scaffolding Enhances the Stability of Suspended Lipid Bilayers for Ion Channel Recordings and Biosensor Development

AU - Bright, Leonard K.

AU - Baker, Christopher A.

AU - Bränström, Robert

AU - Saavedra, Steven S

AU - Aspinwall, Craig A

PY - 2015/10/12

Y1 - 2015/10/12

N2 - Black lipid membranes (BLMs) provide a synthetic environment that facilitates measurement of ion channel activity in diverse analytical platforms. The limited electrical, mechanical and temporal stabilities of BLMs pose a significant challenge to development of highly stable measurement platforms. Here, ethylene glycol dimethacrylate (EGDMA) and butyl methacrylate (BMA) were partitioned into BLMs and photopolymerized to create a cross-linked polymer scaffold in the bilayer lamella that dramatically improved BLM stability. The commercially available methacrylate monomers provide a simple, low cost, and broadly accessible approach for preparing highly stabilized BLMs useful for ion channel-functionalized analytical platforms. When prepared on silane-modified glass microapertures, the resulting polymer scaffold-stabilized (PSS)-BLMs exhibited significantly improved lifetimes of 23 ± 9 to 40 ± 14 h and >10-fold increase in mechanical stability, with breakdown potentials >2000 mV attainable, depending on surface modification and polymer cross-link density. Additionally, the polymer scaffold exerted minimal perturbation to membrane electrical integrity as indicated by mean conductance measurements. When gramicidin A and α-hemolysin were reconstituted into PSS-BLMs, the ion channels retained function comparable to conventional BLMs. This approach is a key advance in the formation of stabilized BLMs and should be amenable to a wide range of receptor and ion channel functionalized platforms.

AB - Black lipid membranes (BLMs) provide a synthetic environment that facilitates measurement of ion channel activity in diverse analytical platforms. The limited electrical, mechanical and temporal stabilities of BLMs pose a significant challenge to development of highly stable measurement platforms. Here, ethylene glycol dimethacrylate (EGDMA) and butyl methacrylate (BMA) were partitioned into BLMs and photopolymerized to create a cross-linked polymer scaffold in the bilayer lamella that dramatically improved BLM stability. The commercially available methacrylate monomers provide a simple, low cost, and broadly accessible approach for preparing highly stabilized BLMs useful for ion channel-functionalized analytical platforms. When prepared on silane-modified glass microapertures, the resulting polymer scaffold-stabilized (PSS)-BLMs exhibited significantly improved lifetimes of 23 ± 9 to 40 ± 14 h and >10-fold increase in mechanical stability, with breakdown potentials >2000 mV attainable, depending on surface modification and polymer cross-link density. Additionally, the polymer scaffold exerted minimal perturbation to membrane electrical integrity as indicated by mean conductance measurements. When gramicidin A and α-hemolysin were reconstituted into PSS-BLMs, the ion channels retained function comparable to conventional BLMs. This approach is a key advance in the formation of stabilized BLMs and should be amenable to a wide range of receptor and ion channel functionalized platforms.

KW - black lipid membrane

KW - gramicidin

KW - ion channel

KW - lipid bilayer

KW - methacrylate polymer

KW - polymer scaffold

KW - α-hemolysin

UR - http://www.scopus.com/inward/record.url?scp=84991523727&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991523727&partnerID=8YFLogxK

U2 - 10.1021/acsbiomaterials.5b00205

DO - 10.1021/acsbiomaterials.5b00205

M3 - Article

AN - SCOPUS:84991523727

VL - 1

SP - 955

EP - 963

JO - ACS Biomaterials Science and Engineering

JF - ACS Biomaterials Science and Engineering

SN - 2373-9878

IS - 10

ER -