Methanogenesis in granular sludge exposed to oxygen

Mario T. Kato, James A Field, Gatze Lettinga

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Substrate competition between methanogenic and facultative bacteria under highly aerobic conditions was investigated in batch experiments. Natural mixed cultures of anaerobic bacteria immobilized in granular sludge were able to concurrently utilize oxygen and produce methane when supplied with ethanol as substrate. The most oxygen tolerant sludge converted 3 to 25% of substrate chemical oxygen demand to methane after 3 days while 23 to 2 mg 1-1 of dissolved oxygen were present in the media. The tolerance of methanogens to oxygen and their coexistence with facultative bacteria were evident even after long periods of oxygen exposure. Eventually, methane oxidizing bacteria developed in the co-culture. The consumption of oxygen by facultative bacteria, creating anaerobic microniches inside the granules, is hypothesized to protect the methanogens.

Original languageEnglish (US)
Pages (from-to)317-323
Number of pages7
JournalFEMS Microbiology Letters
Volume114
Issue number3
StatePublished - Dec 15 1993
Externally publishedYes

Fingerprint

Sewage
Oxygen
Anaerobic Bacteria
Methane
Methylococcaceae
Bacteria
Biological Oxygen Demand Analysis
Coculture Techniques
Oxygen Consumption
Ethanol

Keywords

  • Anaerobic methanogenic and aerobic facultative bacteria
  • Co-culture
  • Methanogens
  • Substrate competition

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Methanogenesis in granular sludge exposed to oxygen. / Kato, Mario T.; Field, James A; Lettinga, Gatze.

In: FEMS Microbiology Letters, Vol. 114, No. 3, 15.12.1993, p. 317-323.

Research output: Contribution to journalArticle

Kato, MT, Field, JA & Lettinga, G 1993, 'Methanogenesis in granular sludge exposed to oxygen', FEMS Microbiology Letters, vol. 114, no. 3, pp. 317-323.
Kato, Mario T. ; Field, James A ; Lettinga, Gatze. / Methanogenesis in granular sludge exposed to oxygen. In: FEMS Microbiology Letters. 1993 ; Vol. 114, No. 3. pp. 317-323.
@article{76a99e07621846df9d12b74a67db7034,
title = "Methanogenesis in granular sludge exposed to oxygen",
abstract = "Substrate competition between methanogenic and facultative bacteria under highly aerobic conditions was investigated in batch experiments. Natural mixed cultures of anaerobic bacteria immobilized in granular sludge were able to concurrently utilize oxygen and produce methane when supplied with ethanol as substrate. The most oxygen tolerant sludge converted 3 to 25{\%} of substrate chemical oxygen demand to methane after 3 days while 23 to 2 mg 1-1 of dissolved oxygen were present in the media. The tolerance of methanogens to oxygen and their coexistence with facultative bacteria were evident even after long periods of oxygen exposure. Eventually, methane oxidizing bacteria developed in the co-culture. The consumption of oxygen by facultative bacteria, creating anaerobic microniches inside the granules, is hypothesized to protect the methanogens.",
keywords = "Anaerobic methanogenic and aerobic facultative bacteria, Co-culture, Methanogens, Substrate competition",
author = "Kato, {Mario T.} and Field, {James A} and Gatze Lettinga",
year = "1993",
month = "12",
day = "15",
language = "English (US)",
volume = "114",
pages = "317--323",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Methanogenesis in granular sludge exposed to oxygen

AU - Kato, Mario T.

AU - Field, James A

AU - Lettinga, Gatze

PY - 1993/12/15

Y1 - 1993/12/15

N2 - Substrate competition between methanogenic and facultative bacteria under highly aerobic conditions was investigated in batch experiments. Natural mixed cultures of anaerobic bacteria immobilized in granular sludge were able to concurrently utilize oxygen and produce methane when supplied with ethanol as substrate. The most oxygen tolerant sludge converted 3 to 25% of substrate chemical oxygen demand to methane after 3 days while 23 to 2 mg 1-1 of dissolved oxygen were present in the media. The tolerance of methanogens to oxygen and their coexistence with facultative bacteria were evident even after long periods of oxygen exposure. Eventually, methane oxidizing bacteria developed in the co-culture. The consumption of oxygen by facultative bacteria, creating anaerobic microniches inside the granules, is hypothesized to protect the methanogens.

AB - Substrate competition between methanogenic and facultative bacteria under highly aerobic conditions was investigated in batch experiments. Natural mixed cultures of anaerobic bacteria immobilized in granular sludge were able to concurrently utilize oxygen and produce methane when supplied with ethanol as substrate. The most oxygen tolerant sludge converted 3 to 25% of substrate chemical oxygen demand to methane after 3 days while 23 to 2 mg 1-1 of dissolved oxygen were present in the media. The tolerance of methanogens to oxygen and their coexistence with facultative bacteria were evident even after long periods of oxygen exposure. Eventually, methane oxidizing bacteria developed in the co-culture. The consumption of oxygen by facultative bacteria, creating anaerobic microniches inside the granules, is hypothesized to protect the methanogens.

KW - Anaerobic methanogenic and aerobic facultative bacteria

KW - Co-culture

KW - Methanogens

KW - Substrate competition

UR - http://www.scopus.com/inward/record.url?scp=0027364789&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027364789&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0027364789

VL - 114

SP - 317

EP - 323

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 3

ER -