Methanogenic inhibition by roxarsone (4-hydroxy-3-nitrophenylarsonic acid) and related aromatic arsenic compounds

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39 Scopus citations

Abstract

Roxarsone (4-hydroxy-3-nitro-phenylarsonic acid) and p-arsanilic acid (4-aminophenylarsonic acid) are feed additives widely used in the broiler and swine industry. This study evaluated the inhibitory effect of roxarsone, p-arsanilic, and other phenylarsonic compounds on the activity of acetate- and H2-utilizing methanogenic microorganisms. Roxarsone, p-arsanilic, and 4-hydroxy-3-aminophenylarsonic acid (HAPA) inhibited acetoclastic and hydrogenotrophic methanogens when supplemented at concentrations of 1 mM, and their inhibitory effect increased sharply with incubation time. Phenylarsonic acid (1 mM) inhibited acetoclastic but not H2-utilizing methanogens. HAPA, a metabolite from the anaerobic biodegradation of roxarsone, was found to be sensitive to autooxidation by oxygen. The compound (2.6 mM) caused low methanogenic inhibition (only 14.2%) in short-term assays of 12 h when autooxidation was prevented by supplementing HAPA solutions with ascorbate. However, ascorbate-free HAPA solutions underwent spontaneous autooxidation in the presence of oxygen, leading to the formation of highly inhibitory compounds. These results confirm the microbial toxicity of organoarsenic compounds, and they indicate that biotic as well as abiotic transformations can potentially impact the fate and microbial toxicity of these contaminants in the environment.

Original languageEnglish (US)
Pages (from-to)352-358
Number of pages7
JournalJournal of Hazardous Materials
Volume175
Issue number1-3
DOIs
StatePublished - Mar 15 2010

Keywords

  • 4-Hydroxy-3-aminophenylarsonic acid
  • Arsenic
  • Methanogenic inhibition
  • Organoarsenic compounds
  • Roxarsone
  • p-Arsanilic acid

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis

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