Methods for concentrating viruses from large volumes of estuarine water on pleated membranes

P. Payment, C. P. Gerba, C. Wallis, J. L. Melnick

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The efficiency of cartridge filters with pleated membranes was determined for the recovery of poliovirus from large volumes of seawater. Flow rates of about 6 gal min-1 were obtained when a combination of a 3- and a 0.45-μm pleated filter were incorporated into the Wallis-Melnick virus concentrator. The filters adsorbed poliovirus when water was acidified at pH 3.5 and aluminum chloride added at a final concentration of 0.0015 m. The filters retained their capacity to adsorb virus particles, even after 600 gal (22681.) of very turbid estuarine water had been filtered. Viruses were eluted from the pleated filters with pH 11.5 glycine buffer and reconcentrated by precipitation with ferric chloride or aluminum chloride. Virus from 100-gal samples was concentrated into a final eluate volume of 20-100 ml, with virus recovery of about 50% being achieved in the material that had been concentrated 20,000-100,000 times.

Original languageEnglish (US)
Pages (from-to)893-896
Number of pages4
JournalWater research
Volume10
Issue number10
DOIs
StatePublished - 1976
Externally publishedYes

ASJC Scopus subject areas

  • Ecological Modeling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

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