Methods for determining chemosensitivity of MCF-7 cells in double-layer agar culture: Labeling index depression and colony count inhibition

D. L. Elson, C. K. Osborne, R. B. Livingston, D. D. Von Hoff

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

An autoradiographic technique has been developed to determine the percentage of MCF-7 human breast adenocarcinoma cells in double-layer agar (DLA) culture that exhibit nuclear incorporation of 3H-thymidine (3H-TdR) after in vitro drug exposure. Depression of this percentage, termed the labeling index, correlated closely with colony count inhibition - relative to the respective control values - for cultures exposed to each of several agents including varying concentrations of 5-fluorouracil and adriamycin. If a similar autoradiographic technique can be applied to cultures from human tumor biopsy specimens, this may then lead to a clinically useful chemosensitivity assay especially applicable when DLA-cultured cell quantities are small.

Original languageEnglish (US)
Pages (from-to)34-44
Number of pages11
JournalStem Cells
Volume2
Issue number1
StatePublished - Dec 1 1982

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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