Methods in laboratory investigation. An ultrastructural cytochemical stain specific for neuroendocrine neoplasms

C. M. Payne, Raymond B Nagle, V. Borduin

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Previous work from our laboratory has indicated that the uranaffin reaction, when run under specific conditions, will stain neurosecretory granules. In this ultrastructural cytochemical study, we analyzed the granule-staining properties of 13 normal, 10 abnormal (non-neoplastic), and 138 neoplastic tissues in an attempt to evaluate the specificity of the uranaffin reaction for diagnostic purposes when compared with routinely processed specimens. For the uranaffin reaction, previously fixed tissue stored in buffer was rinsed with 0.9% NaCl and reacted with a 4% aqueous and processed for electron microscopy. The granules of normal or non-neoplastic neuroendocrine cells that stained positively with the uranaffin reaction included pancreatic islet cells, thyroid C cells, adrenal medullary cells, parathyroid chief cells, and the neuroendocrine cells of the intestine. All 42 neuroendocrine neoplasms studied possessed abundant uranaffin-positive granules and included carcinoids, oat cell carcinomas, islet cell neoplasms, medullary carcinomas of the thyroid, pheochromocytomas, carotid body paragangliomas, a pituitary adenoma, Merkel cell carcinomas, parathyroid adenomas, and a neuroblastoma. All 96 control neoplasms that were not classified as neuroendocrine in nature were negative for neurosecretory granules when studied with the uranaffin reaction and included 13 neoplasms derived from endocrine glands, 57 neoplasms from secretory epithelium, 10 of hematopoietic origin, and 16 miscellaneous neoplasms. It was determined that the uranaffin reaction is a useful ultrastructural cytochemical marker for neuroendocrine granules and helped distinguish these cytoplasmic organelles from ultrastructurally similar granules derived from non-neuroendocrine cells.

Original languageEnglish (US)
Pages (from-to)350-365
Number of pages16
JournalLaboratory Investigation
Volume51
Issue number3
StatePublished - 1984

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Coloring Agents
Islets of Langerhans
Neuroendocrine Cells
Neoplasms
Endocrine Gland Neoplasms
Carotid Body Tumor
Merkel Cell Carcinoma
Parathyroid Neoplasms
Small Cell Carcinoma
Carcinoid Tumor
Pituitary Neoplasms
Pheochromocytoma
Neuroblastoma
Organelles
Intestines
Electron Microscopy
Buffers
Thyroid Gland
Epithelium
Staining and Labeling

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Methods in laboratory investigation. An ultrastructural cytochemical stain specific for neuroendocrine neoplasms. / Payne, C. M.; Nagle, Raymond B; Borduin, V.

In: Laboratory Investigation, Vol. 51, No. 3, 1984, p. 350-365.

Research output: Contribution to journalArticle

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