Mice with a targeted disruption of the AE2 Cl-/HCO 3- exchanger are achlorhydric

Lara R. Gawenis, Clara Ledoussal, Louise M. Judd, Vikram Prasad, Seth L. Alper, Alan Stuart-Tilley, Alison L. Woo, Christina Grisham, L. Philip Sanford, Thomas C Doetschman, Marian L. Miller, Gary E. Shull

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The AE2 Cl-/HCO3- exchanger is expressed in numerous cell types, including epithelial cells of the kidney, respiratory tract, and alimentary tract. In gastric epithelia, AE2 is particularly abundant in parietal cells, where it may be the predominant mechanism for HCO 3- efflux and Cl- influx across the basolateral membrane that is needed for acid secretion. To investigate the hypothesis that AE2 is critical for parietal cell function and to assess its importance in other tissues, homozygous null mutant (AE2-/-) mice were prepared by targeted disruption of the AE2 (Slc4a2) gene. AE2-/- mice were emaciated, edentulous (toothless), and exhibited severe growth retardation, and most of them died around the time of weaning. AE2-/- mice exhibited achlorhydria, and histological studies revealed abnormalities of the gastric epithelium, including moderate dilation of the gastric gland lumens and a reduction in the number of parietal cells. There was little evidence, however, that parietal cell viability was impaired. Ultrastructural analysis of AE2 -/- gastric mucosa revealed abnormal parietal cell structure, with severely impaired development of secretory canaliculi and few tubulovesicles but normal apical microvilli. These results demonstrate that AE2 is essential for gastric acid secretion and for normal development of secretory canalicular and tubulovesicular membranes in mouse parietal cells.

Original languageEnglish (US)
Pages (from-to)30531-30539
Number of pages9
JournalJournal of Biological Chemistry
Volume279
Issue number29
DOIs
StatePublished - Jul 16 2004
Externally publishedYes

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Chloride-Bicarbonate Antiporters
Membranes
Acids
Gastric Mucosa
Genes
Cells
Tissue
Stomach
Epithelium
Achlorhydria
Gastric Acid
Microvilli
Weaning
Respiratory System
Dilatation
Cell Survival
Cell Count
Epithelial Cells
Kidney
Growth

ASJC Scopus subject areas

  • Biochemistry

Cite this

Gawenis, L. R., Ledoussal, C., Judd, L. M., Prasad, V., Alper, S. L., Stuart-Tilley, A., ... Shull, G. E. (2004). Mice with a targeted disruption of the AE2 Cl-/HCO 3- exchanger are achlorhydric. Journal of Biological Chemistry, 279(29), 30531-30539. https://doi.org/10.1074/jbc.M403779200

Mice with a targeted disruption of the AE2 Cl-/HCO 3- exchanger are achlorhydric. / Gawenis, Lara R.; Ledoussal, Clara; Judd, Louise M.; Prasad, Vikram; Alper, Seth L.; Stuart-Tilley, Alan; Woo, Alison L.; Grisham, Christina; Sanford, L. Philip; Doetschman, Thomas C; Miller, Marian L.; Shull, Gary E.

In: Journal of Biological Chemistry, Vol. 279, No. 29, 16.07.2004, p. 30531-30539.

Research output: Contribution to journalArticle

Gawenis, LR, Ledoussal, C, Judd, LM, Prasad, V, Alper, SL, Stuart-Tilley, A, Woo, AL, Grisham, C, Sanford, LP, Doetschman, TC, Miller, ML & Shull, GE 2004, 'Mice with a targeted disruption of the AE2 Cl-/HCO 3- exchanger are achlorhydric', Journal of Biological Chemistry, vol. 279, no. 29, pp. 30531-30539. https://doi.org/10.1074/jbc.M403779200
Gawenis LR, Ledoussal C, Judd LM, Prasad V, Alper SL, Stuart-Tilley A et al. Mice with a targeted disruption of the AE2 Cl-/HCO 3- exchanger are achlorhydric. Journal of Biological Chemistry. 2004 Jul 16;279(29):30531-30539. https://doi.org/10.1074/jbc.M403779200
Gawenis, Lara R. ; Ledoussal, Clara ; Judd, Louise M. ; Prasad, Vikram ; Alper, Seth L. ; Stuart-Tilley, Alan ; Woo, Alison L. ; Grisham, Christina ; Sanford, L. Philip ; Doetschman, Thomas C ; Miller, Marian L. ; Shull, Gary E. / Mice with a targeted disruption of the AE2 Cl-/HCO 3- exchanger are achlorhydric. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 29. pp. 30531-30539.
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