Microcolinearity and genome evolution in the AdhA region of diploid and polyploid cotton (Gossypium)

Corrinne E. Grover, Hyeran Kim, Rod A Wing, Andrew H. Paterson, Jonathan F. Wendel

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Genome sizes vary by several orders of magnitude, driven by mechanisms such as illegitimate recombination and transposable element proliferation. Prior analysis of the CesA region in two cotton genomes that diverged 5-10 million years ago (Ma), and acquired a twofold difference in genome size, revealed extensive local conservation of genic and intergenic regions, with no evidence of the global genome size difference. The present study extends the comparison to include BAC sequences surrounding the gene encoding alcohol dehydrogenase A (AdhA) from four cotton genomes: the two co-resident genomes (AT and DT) of the allotetraploid, Gossypium hirsutum, as well as the model diploid progenitors, Gossypium arboreum (A) and Gossypium raimondii (D). In contrast to earlier work, evolution in the AdhA region reflects, in a microcosm, the overall difference in genome size, with a nearly twofold difference in aligned sequence length. Most size differences may be attributed to differential accumulation of retroelements during divergence of the genome diploids from their common ancestor, but in addition there has been a biased accumulation of small deletions, such that those in the smaller D genome are on average twice as large as those in the larger A genome. The data also provide evidence for the global phenomenon of 'genomic downsizing' in polyploids shortly after formation. This in part reflects a higher frequency of small deletions post-polyploidization, and increased illegitimate recombination. In conjunction with previous work, the data here confirm the conclusion that genome size evolution reflects many forces that collectively operate heterogeneously among genomic regions.

Original languageEnglish (US)
Pages (from-to)995-1006
Number of pages12
JournalPlant Journal
Volume50
Issue number6
DOIs
StatePublished - Jun 2007

Fingerprint

Gossypium
Polyploidy
Alcohol Dehydrogenase
alcohol dehydrogenase
Genome Size
Diploidy
polyploidy
diploidy
cotton
Genome
genome
Genetic Recombination
Retroelements
Intergenic DNA
DNA Transposable Elements
Gossypium raimondii
Gossypium arboreum
genomics
retrotransposons
intergenic DNA

Keywords

  • C-value
  • Cotton
  • Genome evolution
  • Genome size
  • Gossypium
  • Transposable elements

ASJC Scopus subject areas

  • Plant Science

Cite this

Microcolinearity and genome evolution in the AdhA region of diploid and polyploid cotton (Gossypium). / Grover, Corrinne E.; Kim, Hyeran; Wing, Rod A; Paterson, Andrew H.; Wendel, Jonathan F.

In: Plant Journal, Vol. 50, No. 6, 06.2007, p. 995-1006.

Research output: Contribution to journalArticle

Grover, Corrinne E. ; Kim, Hyeran ; Wing, Rod A ; Paterson, Andrew H. ; Wendel, Jonathan F. / Microcolinearity and genome evolution in the AdhA region of diploid and polyploid cotton (Gossypium). In: Plant Journal. 2007 ; Vol. 50, No. 6. pp. 995-1006.
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