Microfluidic-based biosensors toward point-of-care detection of nucleic acids and proteins

Seokheun Choi, Michael Goryll, Lai Yi Mandy Sin, Pak Kin Wong, Junseok Chae

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

This article reviews state-of-the-art microfluidic biosensors of nucleic acids and proteins for point-of-care (POC) diagnostics. Microfluidics is capable of analyzing small sample volumes (10-9-10-18 l) and minimizing costly reagent consumption as well as automating sample preparation and reducing processing time. The merger of microfluidics and advanced biosensor technologies offers new promises for POC diagnostics, including high-throughput analysis, portability and disposability. However, this merger also imposes technological challenges on biosensors, such as high sensitivity and selectivity requirements with sample volumes orders of magnitude smaller than those of conventional practices, false response errors due to non-specific adsorption, and integrability with other necessary modules. There have been many prior review articles on microfluidic-based biosensors, and this review focuses on the recent progress in last 5 years. Herein, we review general technologies of DNA and protein biosensors. Then, recent advances on the coupling of the biosensors to microfluidics are highlighted. Finally, we discuss the key challenges and potential solutions for transforming microfluidic biosensors into POC diagnostic applications.

Original languageEnglish (US)
Pages (from-to)231-247
Number of pages17
JournalMicrofluidics and Nanofluidics
Volume10
Issue number2
DOIs
StatePublished - Feb 2011

Fingerprint

Nucleic acids
nucleic acids
bioinstrumentation
Microfluidics
Biosensors
Nucleic Acids
proteins
Proteins
Disposability
reagents
DNA
deoxyribonucleic acid
modules
selectivity
Throughput
Adsorption
requirements
preparation
adsorption
sensitivity

Keywords

  • Biosensor
  • DNA
  • Microfluidics
  • Point-of-care detection
  • Protein

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry

Cite this

Microfluidic-based biosensors toward point-of-care detection of nucleic acids and proteins. / Choi, Seokheun; Goryll, Michael; Sin, Lai Yi Mandy; Wong, Pak Kin; Chae, Junseok.

In: Microfluidics and Nanofluidics, Vol. 10, No. 2, 02.2011, p. 231-247.

Research output: Contribution to journalArticle

Choi, Seokheun ; Goryll, Michael ; Sin, Lai Yi Mandy ; Wong, Pak Kin ; Chae, Junseok. / Microfluidic-based biosensors toward point-of-care detection of nucleic acids and proteins. In: Microfluidics and Nanofluidics. 2011 ; Vol. 10, No. 2. pp. 231-247.
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