Abstract
This article reviews state-of-the-art microfluidic biosensors of nucleic acids and proteins for point-of-care (POC) diagnostics. Microfluidics is capable of analyzing small sample volumes (10-9-10-18 l) and minimizing costly reagent consumption as well as automating sample preparation and reducing processing time. The merger of microfluidics and advanced biosensor technologies offers new promises for POC diagnostics, including high-throughput analysis, portability and disposability. However, this merger also imposes technological challenges on biosensors, such as high sensitivity and selectivity requirements with sample volumes orders of magnitude smaller than those of conventional practices, false response errors due to non-specific adsorption, and integrability with other necessary modules. There have been many prior review articles on microfluidic-based biosensors, and this review focuses on the recent progress in last 5 years. Herein, we review general technologies of DNA and protein biosensors. Then, recent advances on the coupling of the biosensors to microfluidics are highlighted. Finally, we discuss the key challenges and potential solutions for transforming microfluidic biosensors into POC diagnostic applications.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 231-247 |
| Number of pages | 17 |
| Journal | Microfluidics and Nanofluidics |
| Volume | 10 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2011 |
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Keywords
- Biosensor
- DNA
- Microfluidics
- Point-of-care detection
- Protein
ASJC Scopus subject areas
- Condensed Matter Physics
- Electronic, Optical and Magnetic Materials
- Materials Chemistry
Cite this
Microfluidic-based biosensors toward point-of-care detection of nucleic acids and proteins. / Choi, Seokheun; Goryll, Michael; Sin, Lai Yi Mandy; Wong, Pak Kin; Chae, Junseok.
In: Microfluidics and Nanofluidics, Vol. 10, No. 2, 02.2011, p. 231-247.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microfluidic-based biosensors toward point-of-care detection of nucleic acids and proteins
AU - Choi, Seokheun
AU - Goryll, Michael
AU - Sin, Lai Yi Mandy
AU - Wong, Pak Kin
AU - Chae, Junseok
PY - 2011/2
Y1 - 2011/2
N2 - This article reviews state-of-the-art microfluidic biosensors of nucleic acids and proteins for point-of-care (POC) diagnostics. Microfluidics is capable of analyzing small sample volumes (10-9-10-18 l) and minimizing costly reagent consumption as well as automating sample preparation and reducing processing time. The merger of microfluidics and advanced biosensor technologies offers new promises for POC diagnostics, including high-throughput analysis, portability and disposability. However, this merger also imposes technological challenges on biosensors, such as high sensitivity and selectivity requirements with sample volumes orders of magnitude smaller than those of conventional practices, false response errors due to non-specific adsorption, and integrability with other necessary modules. There have been many prior review articles on microfluidic-based biosensors, and this review focuses on the recent progress in last 5 years. Herein, we review general technologies of DNA and protein biosensors. Then, recent advances on the coupling of the biosensors to microfluidics are highlighted. Finally, we discuss the key challenges and potential solutions for transforming microfluidic biosensors into POC diagnostic applications.
AB - This article reviews state-of-the-art microfluidic biosensors of nucleic acids and proteins for point-of-care (POC) diagnostics. Microfluidics is capable of analyzing small sample volumes (10-9-10-18 l) and minimizing costly reagent consumption as well as automating sample preparation and reducing processing time. The merger of microfluidics and advanced biosensor technologies offers new promises for POC diagnostics, including high-throughput analysis, portability and disposability. However, this merger also imposes technological challenges on biosensors, such as high sensitivity and selectivity requirements with sample volumes orders of magnitude smaller than those of conventional practices, false response errors due to non-specific adsorption, and integrability with other necessary modules. There have been many prior review articles on microfluidic-based biosensors, and this review focuses on the recent progress in last 5 years. Herein, we review general technologies of DNA and protein biosensors. Then, recent advances on the coupling of the biosensors to microfluidics are highlighted. Finally, we discuss the key challenges and potential solutions for transforming microfluidic biosensors into POC diagnostic applications.
KW - Biosensor
KW - DNA
KW - Microfluidics
KW - Point-of-care detection
KW - Protein
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UR - http://www.scopus.com/inward/citedby.url?scp=79551486836&partnerID=8YFLogxK
U2 - 10.1007/s10404-010-0638-8
DO - 10.1007/s10404-010-0638-8
M3 - Article
AN - SCOPUS:79551486836
VL - 10
SP - 231
EP - 247
JO - Microfluidics and Nanofluidics
JF - Microfluidics and Nanofluidics
SN - 1613-4982
IS - 2
ER -