Microfluidic device detection of waterborne pathogens through static light scattering of latex immunoagglutination using proximity optical fibers

Jeong-Yeol Yoon, Jin Hee Han, Brian Heinze, Lonnie J. Lucas

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Microfluidic device detections of E. coli K12 in deionized (DI) water and E. coli in field water sample were demonstrated through static light scattering of latex immunoagglutination using proximity optical fibers. This method is a fully-automated, one-step detection, and requires neither sample pre-treatment nor cell culturing often required in many on-chip detections. We have used highly carboxylated polystyrene submicron latex particles without surfactants to enhance diffusional mixing and prevent non-specific bindings towards successful demonstration of latex immunoagglutination in microfluidic device. Detection of E. coli was performed by taking microscopic images from the view cell of a microfluidic device and counting the fractions of non-agglutinated and agglutinated particles. The limit of detection (LOD) was ca. 150 CFU ml -1 with this method for both E. coli K12 in DI water and E. coli in field water sample, indicating no non-specific bindings. Improved LOD of < 4.3 CFU ml-1 was achieved by measuring forward static light scattering from microfluidic device, using proximity optical fibers and a USB-powered miniature spectrometer. The total assay time for sample preparation (mostly dilutions) and on-chip assay (mostly injections and short incubation time) was < 10 min.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Volume6556
DOIs
StatePublished - 2007
EventMicro (MEMS) and Nanotechnologies for Defense and Security - Orlando, FL, United States
Duration: Apr 10 2007Apr 12 2007

Other

OtherMicro (MEMS) and Nanotechnologies for Defense and Security
CountryUnited States
CityOrlando, FL
Period4/10/074/12/07

Fingerprint

pathogens
microfluidic devices
Pathogens
latex
Latexes
Microfluidics
Light scattering
Escherichia coli
proximity
Optical fibers
light scattering
optical fibers
Deionized water
Assays
water
cell culturing
chips
Particles (particulate matter)
Dilution
Spectrometers

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Yoon, J-Y., Han, J. H., Heinze, B., & Lucas, L. J. (2007). Microfluidic device detection of waterborne pathogens through static light scattering of latex immunoagglutination using proximity optical fibers. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 6556). [65560M] https://doi.org/10.1117/12.721129

Microfluidic device detection of waterborne pathogens through static light scattering of latex immunoagglutination using proximity optical fibers. / Yoon, Jeong-Yeol; Han, Jin Hee; Heinze, Brian; Lucas, Lonnie J.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 6556 2007. 65560M.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yoon, J-Y, Han, JH, Heinze, B & Lucas, LJ 2007, Microfluidic device detection of waterborne pathogens through static light scattering of latex immunoagglutination using proximity optical fibers. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 6556, 65560M, Micro (MEMS) and Nanotechnologies for Defense and Security, Orlando, FL, United States, 4/10/07. https://doi.org/10.1117/12.721129
Yoon J-Y, Han JH, Heinze B, Lucas LJ. Microfluidic device detection of waterborne pathogens through static light scattering of latex immunoagglutination using proximity optical fibers. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 6556. 2007. 65560M https://doi.org/10.1117/12.721129
Yoon, Jeong-Yeol ; Han, Jin Hee ; Heinze, Brian ; Lucas, Lonnie J. / Microfluidic device detection of waterborne pathogens through static light scattering of latex immunoagglutination using proximity optical fibers. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 6556 2007.
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