Microfluidic immunosensor for rapid and sensitive detection of bovine viral diarrhea virus

Brian C. Heinze, Jae Young Song, Chang Hee Lee, Anbar Najam, Jeong-Yeol Yoon

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We have investigated the utilization of microparticle immunoagglutination assays using forward light scattering measurements in a microfluidic chip towards detecting viral particles. The model viral target was bovine viral diarrhea virus (BVDV). Highly carboxylated polystyrene microparticles (510 nm) were coated with anti-BVDV monoclonal antibodies. This solution was in turn used to detect BVDV in diluted tissue culture media and fetal calf serum. The assay was first performed in a two well slide format for proof of concept and particle stability experiments, then in a simple y-channel microfluidic chip with optical fibers arranged in a close proximity setup. Microparticle immunoagglutination was detected via static forward light scattering measurements taken at 45° to incident light. In the microfluidic chip, BVDV was detected down to a concentration of 10 TCID50 mL-1. For assay comparison purposes reverse transcriptase polymerase chain reaction (RT-PCR) was performed on serial dilutions of the BVDV sample used in the two well and microfluidic testing. RT-PCR was effective down to a concentration of 103 TCID50 mL-1 in detecting the identical BVDV used for microfluidic assays.

Original languageEnglish (US)
Pages (from-to)491-496
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume138
Issue number2
DOIs
StatePublished - May 6 2009

Fingerprint

Immunosensors
viruses
Viruses
Microfluidics
Assays
microparticles
Forward scattering
polymerase chain reaction
Polymerase chain reaction
RNA-Directed DNA Polymerase
chips
forward scattering
Light scattering
light scattering
Tissue culture
culture media
calves
Monoclonal antibodies
Polystyrenes
antibodies

Keywords

  • Biosensor
  • BVDV
  • Immunoassay
  • Lab-on-a-chip
  • Static light scattering
  • Virus detection

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Microfluidic immunosensor for rapid and sensitive detection of bovine viral diarrhea virus. / Heinze, Brian C.; Song, Jae Young; Lee, Chang Hee; Najam, Anbar; Yoon, Jeong-Yeol.

In: Sensors and Actuators, B: Chemical, Vol. 138, No. 2, 06.05.2009, p. 491-496.

Research output: Contribution to journalArticle

Heinze, Brian C. ; Song, Jae Young ; Lee, Chang Hee ; Najam, Anbar ; Yoon, Jeong-Yeol. / Microfluidic immunosensor for rapid and sensitive detection of bovine viral diarrhea virus. In: Sensors and Actuators, B: Chemical. 2009 ; Vol. 138, No. 2. pp. 491-496.
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