Microfluidic immunosensor with integrated liquid core waveguides for sensitive Mie scattering detection of avian influenza antigens in a real biological matrix

Brian C. Heinze, Jessica R. Gamboa, Keesung Kim, Jae Young Song, Jeong-Yeol Yoon

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

This work presents the use of integrated, liquid core, optical waveguides for measuring immunoagglutinationinduced light scattering in a microfluidic device, towards rapid and sensitive detection of avian influenza (AI) viral antigens in a real biological matrix (chicken feces). Mie scattering simulations were performed and tested to optimize the scattering efficiency of the device through proper scatter angle waveguide geometry. The detection limit is demonstrated to be 1 pgmL-1 in both clean buffer and real biological matrix. This low detection limit is made possible through on-chip diffusional mixing of AI target antigens and high acid content microparticle assay reagents, coupledwith real-timemonitoring of immunoagglutination-induced forward Mie scattering via high refractive index liquid core optical waveguides in close proximity (100 μm) to the sample chamber. The detection time for the assay is <2 min. This device could easily be modified to detect trace levels of any biological molecules that antibodies are available for, moving towards a robust platform for pointof- care disease diagnostics.

Original languageEnglish (US)
Pages (from-to)2693-2700
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
Volume398
Issue number6
DOIs
StatePublished - Nov 2010

Fingerprint

Immunosensors
Influenza in Birds
Microfluidics
Optical waveguides
Lab-On-A-Chip Devices
Limit of Detection
Assays
Waveguides
Scattering
Antigens
Forward scattering
Equipment and Supplies
Refractometry
Viral Antigens
Liquids
Feces
Light scattering
Chickens
Refractive index
Buffers

Keywords

  • Bird flu
  • Immunoassay
  • Influenza A
  • Lab-on-a-chip
  • Latex agglutination
  • Optofluidic

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Microfluidic immunosensor with integrated liquid core waveguides for sensitive Mie scattering detection of avian influenza antigens in a real biological matrix. / Heinze, Brian C.; Gamboa, Jessica R.; Kim, Keesung; Song, Jae Young; Yoon, Jeong-Yeol.

In: Analytical and Bioanalytical Chemistry, Vol. 398, No. 6, 11.2010, p. 2693-2700.

Research output: Contribution to journalArticle

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