Microfluidic module for blood cell separation for gene expression radiobiological assays

Muriel Brengues, Jian Gu, Frederic Zenhausern

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Advances in molecular techniques have improved discovery of biomarkers associated with radiation exposure. Gene expression techniques have been demonstrated as effective tools for biodosimetry, and different assay platforms with different chemistries are now available. One of the main challenges is to integrate the sample preparation processing of these assays into microfluidic platforms to be fully automated for point-of-care medical countermeasures in the case of a radiological event. Most of these assays follow the same workflow processing that comprises first the collection of blood samples followed by cellular and molecular sample preparation. The sample preparation is based on the specific reagents of the assay system and depends also on the different subsets of cells population and the type of biomarkers of interest. In this article, the authors present a module for isolation of white blood cells from peripheral blood as a prerequisite for automation of gene expression assays on a microfluidic cartridge. For each sample condition, the gene expression platform can be adapted to suit the requirements of the selected assay chemistry.

Original languageEnglish (US)
Article numberncv138
Pages (from-to)306-310
Number of pages5
JournalRadiation Protection Dosimetry
Volume166
Issue number1-4
DOIs
StatePublished - Sep 1 2015

Fingerprint

blood cells
Microfluidics
Cell Separation
gene expression
Blood Cells
modules
Gene Expression
Biomarkers
Point-of-Care Systems
platforms
biomarkers
Workflow
Automation
preparation
blood
chemistry
Leukocytes
cartridges
leukocytes
countermeasures

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Radiation
  • Public Health, Environmental and Occupational Health

Cite this

Microfluidic module for blood cell separation for gene expression radiobiological assays. / Brengues, Muriel; Gu, Jian; Zenhausern, Frederic.

In: Radiation Protection Dosimetry, Vol. 166, No. 1-4, ncv138, 01.09.2015, p. 306-310.

Research output: Contribution to journalArticle

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