Microscopic Techniques

Timberley M. Roane, Ian L Pepper

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Microscopy was the initial methodology of choice for the study of microorganisms, dating back to the seventeenth century. In this chapter we provide information on all types of microscopy ranging from visible light microscopy to scanning probe microscopy. We begin with a discussion of the theory of microscopy including such key concepts as "resolution," "aberration," "magnification" and "contrast." Various kinds of visible light microscopy are described including: bright-field; dark-field; phase-contrast; differential interference contrast; and polarization microscopy. Fluorescence microscopic techniques include: fluorescence immunolabeling; flow cytometry; and confocal laser scanning. Sections on electron microscopy are also presented, covering scanning and transmission electron microcopy. The chapter concludes with coverage of scanning probe microscopy including atomic force microscopy.

Original languageEnglish (US)
Title of host publicationEnvironmental Microbiology
Subtitle of host publicationThird Edition
PublisherElsevier Inc.
Pages177-193
Number of pages17
ISBN (Print)9780123946263
DOIs
StatePublished - Jan 1 2014

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Microscopy
Microscopic examination
Scanning Probe Microscopy
Scanning probe microscopy
Optical microscopy
Fluorescence
Scanning
Flow cytometry
Polarization Microscopy
Light
Aberrations
Microorganisms
Electron microscopy
Atomic Force Microscopy
Atomic force microscopy
Polarization
Electron Microscopy
Flow Cytometry
Lasers
Electrons

Keywords

  • Electron microscopy
  • Fluorescence microscopy
  • Light microscopy
  • Microscopy
  • Scanning probe microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Roane, T. M., & Pepper, I. L. (2014). Microscopic Techniques. In Environmental Microbiology: Third Edition (pp. 177-193). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-394626-3.00009-0

Microscopic Techniques. / Roane, Timberley M.; Pepper, Ian L.

Environmental Microbiology: Third Edition. Elsevier Inc., 2014. p. 177-193.

Research output: Chapter in Book/Report/Conference proceedingChapter

Roane, TM & Pepper, IL 2014, Microscopic Techniques. in Environmental Microbiology: Third Edition. Elsevier Inc., pp. 177-193. https://doi.org/10.1016/B978-0-12-394626-3.00009-0
Roane TM, Pepper IL. Microscopic Techniques. In Environmental Microbiology: Third Edition. Elsevier Inc. 2014. p. 177-193 https://doi.org/10.1016/B978-0-12-394626-3.00009-0
Roane, Timberley M. ; Pepper, Ian L. / Microscopic Techniques. Environmental Microbiology: Third Edition. Elsevier Inc., 2014. pp. 177-193
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