Microsomal bioactivation and covalent binding of aliphatic halides to DNA

A. B. Direnzo, A Jay Gandolfi, I. G. Sipes

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Studies were carried out on the in vitro covalent binding of a series of 14C-labeled aliphatic halides to calf thymus DNA following bioactivation by hepatic microsomes isolated from phenobarbital-treated rats. Six compounds were shown to exhibit binding to DNA of greater than 0.3 nmol/mg DNA (1,2-dibromoethane, bromotrichloromethane, trichloroethylene, carbon tetrachloride, chloroform, and 1,1,2-trichloroethane). Covalent binding of the aliphatic halides to the nucleic acids was confirmed by sedimentation of the DNA-organohalogen adduct in a cesium chloride gradient and Sephadex LH-20 chromatography of the nucleosides released by enzymatic hydrolysis.

Original languageEnglish (US)
Pages (from-to)243-252
Number of pages10
JournalToxicology Letters
Volume11
Issue number3-4
DOIs
StatePublished - 1982

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Bromotrichloromethane
Ethylene Dibromide
Trichloroethylene
Enzymatic hydrolysis
DNA Adducts
Carbon Tetrachloride
DNA
Phenobarbital
Chloroform
Microsomes
Chromatography
Nucleosides
Sedimentation
Nucleic Acids
Rats
Hydrolysis
Liver
calf thymus DNA
sephadex
cesium chloride

ASJC Scopus subject areas

  • Toxicology

Cite this

Microsomal bioactivation and covalent binding of aliphatic halides to DNA. / Direnzo, A. B.; Gandolfi, A Jay; Sipes, I. G.

In: Toxicology Letters, Vol. 11, No. 3-4, 1982, p. 243-252.

Research output: Contribution to journalArticle

Direnzo, A. B. ; Gandolfi, A Jay ; Sipes, I. G. / Microsomal bioactivation and covalent binding of aliphatic halides to DNA. In: Toxicology Letters. 1982 ; Vol. 11, No. 3-4. pp. 243-252.
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