Microsporidia are natural intracellular parasites of the nematode Caenorhabditis elegans.

Emily R. Troemel, Marie Anne Félix, Noah K Whiteman, Antoine Barrière, Frederick M. Ausubel

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

For decades the soil nematode Caenorhabditis elegans has been an important model system for biology, but little is known about its natural ecology. Recently, C. elegans has become the focus of studies of innate immunity and several pathogens have been shown to cause lethal intestinal infections in C. elegans. However none of these pathogens has been shown to invade nematode intestinal cells, and no pathogen has been isolated from wild-caught C. elegans. Here we describe an intracellular pathogen isolated from wild-caught C. elegans that we show is a new species of microsporidia. Microsporidia comprise a large class of eukaryotic intracellular parasites that are medically and agriculturally important, but poorly understood. We show that microsporidian infection of the C. elegans intestine proceeds through distinct stages and is transmitted horizontally. Disruption of a conserved cytoskeletal structure in the intestine called the terminal web correlates with the release of microsporidian spores from infected cells, and appears to be part of a novel mechanism by which intracellular pathogens exit from infected cells. Unlike in bacterial intestinal infections, the p38 MAPK and insulin/insulin-like growth factor (IGF) signaling pathways do not appear to play substantial roles in resistance to microsporidian infection in C. elegans. We found microsporidia in multiple wild-caught isolates of Caenorhabditis nematodes from diverse geographic locations. These results indicate that microsporidia are common parasites of C. elegans in the wild. In addition, the interaction between C. elegans and its natural microsporidian parasites provides a system in which to dissect intracellular intestinal infection in vivo and insight into the diversity of pathogenic mechanisms used by intracellular microbes.

Original languageEnglish (US)
Article number2736-2752
JournalPLoS Biology
Volume6
Issue number12
StatePublished - Dec 9 2008
Externally publishedYes

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Microsporidia
Caenorhabditis elegans
Pathogens
Parasites
Nematoda
parasites
pathogens
infection
Infection
Somatomedins
p38 Mitogen-Activated Protein Kinases
Ecology
Intestines
intestines
Caenorhabditis
Geographic Locations
Insulin
soil nematodes
Systems Biology
Soils

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Troemel, E. R., Félix, M. A., Whiteman, N. K., Barrière, A., & Ausubel, F. M. (2008). Microsporidia are natural intracellular parasites of the nematode Caenorhabditis elegans. PLoS Biology, 6(12), [2736-2752].

Microsporidia are natural intracellular parasites of the nematode Caenorhabditis elegans. / Troemel, Emily R.; Félix, Marie Anne; Whiteman, Noah K; Barrière, Antoine; Ausubel, Frederick M.

In: PLoS Biology, Vol. 6, No. 12, 2736-2752, 09.12.2008.

Research output: Contribution to journalArticle

Troemel, ER, Félix, MA, Whiteman, NK, Barrière, A & Ausubel, FM 2008, 'Microsporidia are natural intracellular parasites of the nematode Caenorhabditis elegans.', PLoS Biology, vol. 6, no. 12, 2736-2752.
Troemel ER, Félix MA, Whiteman NK, Barrière A, Ausubel FM. Microsporidia are natural intracellular parasites of the nematode Caenorhabditis elegans. PLoS Biology. 2008 Dec 9;6(12). 2736-2752.
Troemel, Emily R. ; Félix, Marie Anne ; Whiteman, Noah K ; Barrière, Antoine ; Ausubel, Frederick M. / Microsporidia are natural intracellular parasites of the nematode Caenorhabditis elegans. In: PLoS Biology. 2008 ; Vol. 6, No. 12.
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