Microsystem-based study of pollen-tube attractants secreted by ovules

J. R. Cooper, Y. Qin, L. Jiang, R. Palanivelu, Y. Zohar

Research output: Contribution to journalConference article

2 Scopus citations

Abstract

Microdevices are developed to resemble the in-vivo micro-environment of ovule fertilization by pollen tubes in model research plant Arabidopsis thaliana. The PDMSbased microdevices are filled with pollen germination medium (PGM) providing pollen tubes with a proper growth environment. Pollen tubes are found to grow within the microgrooves with an average rate of 130tm/hr, reaching a final tube length of about 450tm. Targeting ovules by pollen tubes has also been tested with an observed efficiency of about 67%. Both the pollen tube growth rate and the ovule targeting efficiency in microdevices are similar to those obtained using in-vitro plate experiments. Finally, initial results indicate that pollen tube bundles preferentially turn toward ovule containing chambers, suggesting that the pollen tubes respond to the attractants secreted by unfertilized ovules.

Original languageEnglish (US)
Article number4805355
Pages (from-to)208-211
Number of pages4
JournalProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
DOIs
StatePublished - Jun 1 2009
Event22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009 - Sorrento, Italy
Duration: Jan 25 2009Jan 29 2009

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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