Microsystem-based study of pollen-tube attractants secreted by ovules

J. R. Cooper, Y. Qin, L. Jiang, Ravishankar Palanivelu, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Microdevices are developed to resemble the in-vivo micro-environment of ovule fertilization by pollen tubes in model research plant Arabidopsis thaliana. The PDMSbased microdevices are filled with pollen germination medium (PGM) providing pollen tubes with a proper growth environment. Pollen tubes are found to grow within the microgrooves with an average rate of 130tm/hr, reaching a final tube length of about 450tm. Targeting ovules by pollen tubes has also been tested with an observed efficiency of about 67%. Both the pollen tube growth rate and the ovule targeting efficiency in microdevices are similar to those obtained using in-vitro plate experiments. Finally, initial results indicate that pollen tube bundles preferentially turn toward ovule containing chambers, suggesting that the pollen tubes respond to the attractants secreted by unfertilized ovules.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages208-211
Number of pages4
DOIs
StatePublished - 2009
Event22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009 - Sorrento, Italy
Duration: Jan 25 2009Jan 29 2009

Other

Other22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009
CountryItaly
CitySorrento
Period1/25/091/29/09

Fingerprint

pollen
Microsystems
tubes
Experiments
germination
fertilization
bundles
chambers

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Cooper, J. R., Qin, Y., Jiang, L., Palanivelu, R., & Zohar, Y. (2009). Microsystem-based study of pollen-tube attractants secreted by ovules. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 208-211). [4805355] https://doi.org/10.1109/MEMSYS.2009.4805355

Microsystem-based study of pollen-tube attractants secreted by ovules. / Cooper, J. R.; Qin, Y.; Jiang, L.; Palanivelu, Ravishankar; Zohar, Yitshak.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2009. p. 208-211 4805355.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Cooper, JR, Qin, Y, Jiang, L, Palanivelu, R & Zohar, Y 2009, Microsystem-based study of pollen-tube attractants secreted by ovules. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 4805355, pp. 208-211, 22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009, Sorrento, Italy, 1/25/09. https://doi.org/10.1109/MEMSYS.2009.4805355
Cooper JR, Qin Y, Jiang L, Palanivelu R, Zohar Y. Microsystem-based study of pollen-tube attractants secreted by ovules. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2009. p. 208-211. 4805355 https://doi.org/10.1109/MEMSYS.2009.4805355
Cooper, J. R. ; Qin, Y. ; Jiang, L. ; Palanivelu, Ravishankar ; Zohar, Yitshak. / Microsystem-based study of pollen-tube attractants secreted by ovules. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2009. pp. 208-211
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