Mie Scatter and Interfacial Tension Based Real-Time Quantification of Colloidal Emulsion Nucleic Acid Amplification

Ariana M. Nicolini, Tyler D. Toth, Samuel Y. Kim, M. Alejandra Mandel, David W Galbraith, Jeong-Yeol Yoon

Research output: Contribution to journalArticle

Abstract

This work demonstrates for the first time rapid, real-time Mie scatter sensing of colloidal emulsion nucleic acid amplification directly from emulsion droplets. Loop-mediated isothermal amplification is used in this study, and, to our knowledge, has not previously been used in a colloidal emulsion platform. Interfacial tension values (γ) associated with bulk protein adsorption and denaturation at the oil–water interface exhibit characteristic changes in the absence or presence of amplification. In the presence of target and amplicon, emulsions maintain a constant 300–400 nm diameter, whereas emulsions formed with no target control show a rapid decrease in droplet diameter to <100 nm over the first 20 min of incubation. This method is validated using whole bacteria (Staphylococcus aureus MSSA and Escherichia coli O157:H7) and whole virus (Potato virus Y and Zika virus) samples suspended in water, buffer, or serum-like matrices. Short-term formation of colloidal emulsion is quantified via 60° scatter monitoring, where the initial slope of scattering intensity is utilized to confirm target amplification in less than 5 min. The unique benefits of this method render it more cost-effective and field-deployable than existing methods, while being adaptable to a multitude of targets, sample matrices, and nucleic acid amplification tests.

Original languageEnglish (US)
Article number1700098
JournalAdvanced Biosystems
Volume1
Issue number10
DOIs
StatePublished - Oct 1 2017

Fingerprint

Surface Tension
Nucleic acids
Emulsions
Nucleic Acids
Amplification
Surface tension
Viruses
Nucleic Acid Amplification Techniques
Potyvirus
Protein Denaturation
Denaturation
Escherichia coli O157
Escherichia coli
Adsorption
Staphylococcus aureus
Bacteria
Buffers
Scattering
Proteins
Costs and Cost Analysis

Keywords

  • LAMP
  • loop-mediated isothermal amplification
  • Mie scatter
  • real-time quantification
  • w/o emulsions

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biomaterials
  • Biomedical Engineering

Cite this

Mie Scatter and Interfacial Tension Based Real-Time Quantification of Colloidal Emulsion Nucleic Acid Amplification. / Nicolini, Ariana M.; Toth, Tyler D.; Kim, Samuel Y.; Mandel, M. Alejandra; Galbraith, David W; Yoon, Jeong-Yeol.

In: Advanced Biosystems, Vol. 1, No. 10, 1700098, 01.10.2017.

Research output: Contribution to journalArticle

@article{5e0adc278cff4bfbb1462c681042c5a5,
title = "Mie Scatter and Interfacial Tension Based Real-Time Quantification of Colloidal Emulsion Nucleic Acid Amplification",
abstract = "This work demonstrates for the first time rapid, real-time Mie scatter sensing of colloidal emulsion nucleic acid amplification directly from emulsion droplets. Loop-mediated isothermal amplification is used in this study, and, to our knowledge, has not previously been used in a colloidal emulsion platform. Interfacial tension values (γ) associated with bulk protein adsorption and denaturation at the oil–water interface exhibit characteristic changes in the absence or presence of amplification. In the presence of target and amplicon, emulsions maintain a constant 300–400 nm diameter, whereas emulsions formed with no target control show a rapid decrease in droplet diameter to <100 nm over the first 20 min of incubation. This method is validated using whole bacteria (Staphylococcus aureus MSSA and Escherichia coli O157:H7) and whole virus (Potato virus Y and Zika virus) samples suspended in water, buffer, or serum-like matrices. Short-term formation of colloidal emulsion is quantified via 60° scatter monitoring, where the initial slope of scattering intensity is utilized to confirm target amplification in less than 5 min. The unique benefits of this method render it more cost-effective and field-deployable than existing methods, while being adaptable to a multitude of targets, sample matrices, and nucleic acid amplification tests.",
keywords = "LAMP, loop-mediated isothermal amplification, Mie scatter, real-time quantification, w/o emulsions",
author = "Nicolini, {Ariana M.} and Toth, {Tyler D.} and Kim, {Samuel Y.} and Mandel, {M. Alejandra} and Galbraith, {David W} and Jeong-Yeol Yoon",
year = "2017",
month = "10",
day = "1",
doi = "10.1002/adbi.201700098",
language = "English (US)",
volume = "1",
journal = "Advanced Biosystems",
issn = "2366-7478",
publisher = "Wiley-VCH Verlag",
number = "10",

}

TY - JOUR

T1 - Mie Scatter and Interfacial Tension Based Real-Time Quantification of Colloidal Emulsion Nucleic Acid Amplification

AU - Nicolini, Ariana M.

AU - Toth, Tyler D.

AU - Kim, Samuel Y.

AU - Mandel, M. Alejandra

AU - Galbraith, David W

AU - Yoon, Jeong-Yeol

PY - 2017/10/1

Y1 - 2017/10/1

N2 - This work demonstrates for the first time rapid, real-time Mie scatter sensing of colloidal emulsion nucleic acid amplification directly from emulsion droplets. Loop-mediated isothermal amplification is used in this study, and, to our knowledge, has not previously been used in a colloidal emulsion platform. Interfacial tension values (γ) associated with bulk protein adsorption and denaturation at the oil–water interface exhibit characteristic changes in the absence or presence of amplification. In the presence of target and amplicon, emulsions maintain a constant 300–400 nm diameter, whereas emulsions formed with no target control show a rapid decrease in droplet diameter to <100 nm over the first 20 min of incubation. This method is validated using whole bacteria (Staphylococcus aureus MSSA and Escherichia coli O157:H7) and whole virus (Potato virus Y and Zika virus) samples suspended in water, buffer, or serum-like matrices. Short-term formation of colloidal emulsion is quantified via 60° scatter monitoring, where the initial slope of scattering intensity is utilized to confirm target amplification in less than 5 min. The unique benefits of this method render it more cost-effective and field-deployable than existing methods, while being adaptable to a multitude of targets, sample matrices, and nucleic acid amplification tests.

AB - This work demonstrates for the first time rapid, real-time Mie scatter sensing of colloidal emulsion nucleic acid amplification directly from emulsion droplets. Loop-mediated isothermal amplification is used in this study, and, to our knowledge, has not previously been used in a colloidal emulsion platform. Interfacial tension values (γ) associated with bulk protein adsorption and denaturation at the oil–water interface exhibit characteristic changes in the absence or presence of amplification. In the presence of target and amplicon, emulsions maintain a constant 300–400 nm diameter, whereas emulsions formed with no target control show a rapid decrease in droplet diameter to <100 nm over the first 20 min of incubation. This method is validated using whole bacteria (Staphylococcus aureus MSSA and Escherichia coli O157:H7) and whole virus (Potato virus Y and Zika virus) samples suspended in water, buffer, or serum-like matrices. Short-term formation of colloidal emulsion is quantified via 60° scatter monitoring, where the initial slope of scattering intensity is utilized to confirm target amplification in less than 5 min. The unique benefits of this method render it more cost-effective and field-deployable than existing methods, while being adaptable to a multitude of targets, sample matrices, and nucleic acid amplification tests.

KW - LAMP

KW - loop-mediated isothermal amplification

KW - Mie scatter

KW - real-time quantification

KW - w/o emulsions

UR - http://www.scopus.com/inward/record.url?scp=85065053904&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065053904&partnerID=8YFLogxK

U2 - 10.1002/adbi.201700098

DO - 10.1002/adbi.201700098

M3 - Article

AN - SCOPUS:85065053904

VL - 1

JO - Advanced Biosystems

JF - Advanced Biosystems

SN - 2366-7478

IS - 10

M1 - 1700098

ER -