Migration versus proliferation as contributor to in vitro wound healing of vascular endothelial and smooth muscle cells

Kaitlyn R. Ammann, Katrina J. DeCook, Maxwell Li, Marvin J Slepian

Research output: Contribution to journalArticle

Abstract

Wound closure, as a result of collective cell growth, is an essential biological response to injury. In the field of vascular biology, the response of vascular smooth muscle cells (SMCs) and endothelial cells (ECs) to injury and substrate surface is important in therapeutic clinical treatment interventions such as angioplasty and atherectomy. Specifically, the mechanism by which cells close wounds (i.e. proliferation versus migration) in response to injury stimuli is of interest to better modulate recurrent vascular stenosis, prevent thrombus formation, occlusion, and life-threatening cardiovascular events. Here, we examine growth extent and temporal sequence of events following wound or gap introduction to a confluent monolayer of vascular SMCs or ECs. Significant differences in the preferred mechanisms of these cells to close wounds or gaps were observed; after 48 h, 73% of SMC wound closure was observed to be due to proliferation, while 75% of EC wound closure resulted from migration. These mechanisms were further modulated via addition or removal of extracellular matrix substrate and injury, with ECs more responsive to substrate composition and less to injury, in comparison to SMCs. Our results indicate that ECs and SMCs heal wounds differently, and that the time and mode of injury and associated substrate surface all impact this response.

Original languageEnglish (US)
Pages (from-to)58-66
Number of pages9
JournalExperimental Cell Research
Volume376
Issue number1
DOIs
StatePublished - Mar 1 2019

Fingerprint

Vascular Smooth Muscle
Wound Healing
Smooth Muscle Myocytes
Wounds and Injuries
Endothelial Cells
In Vitro Techniques
Blood Vessels
Atherectomy
Growth
Angioplasty
Extracellular Matrix
Pathologic Constriction
Thrombosis

Keywords

  • Endothelial cells
  • Migration
  • Proliferation
  • Smooth muscle cells
  • Vascular
  • Wound healing

ASJC Scopus subject areas

  • Cell Biology

Cite this

Migration versus proliferation as contributor to in vitro wound healing of vascular endothelial and smooth muscle cells. / Ammann, Kaitlyn R.; DeCook, Katrina J.; Li, Maxwell; Slepian, Marvin J.

In: Experimental Cell Research, Vol. 376, No. 1, 01.03.2019, p. 58-66.

Research output: Contribution to journalArticle

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