Miniature-and multiple-eyespot loci in chlamydomonas reinhardtii define new modulators of eyespot photoreception and assembly

Joseph S. Boyd, Mary Rose Lamb, Carol L Dieckmann

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The photosensory eyespot of the green alga Chlamydomonas reinhardtii is a model system for the study of organelle biogenesis and placement. Eyespot assembly and positioning are governed by several genetic loci that have been identified in forward genetic screens for phototaxis-defective mutants. These include the previously described miniature-eyespot mutant min1, the multiple-eyespot mutant mlt1, the eyeless mutants eye2 and eye3, and two previously uncharacterized eyespot mutants, min2 and mlt2. In this study, effects of miniature-and multiple-eyespot mutations and their combinations on the localization and expression levels of the rhodopsin photoreceptor channelrhodopsin-1 (ChR1) and the localization of the eyespot-assembly proteins EYE2 and EYE3 were examined. min2 mutants assemble a properly organized, albeit nonfunctional, eyespot that is slightly smaller than wild-type; however, combination of the min2 and mlt1 mutations resulted in drastic reduction of photoreceptor levels. Both stationary-phase mlt1 and mlt2 cells have supernumerary, mislocalized eyespots that exhibit partial or total dissociation of the eyespot layers. In these mutant strains, photoreceptor patches in the plasma membrane were never associated with pigment granule arrays in the chloroplast stroma unless EYE2 was present in the intervening envelope. The data suggest that MIN2 is required for the photoreceptive ability of the eyespot and that MLT2 plays a major role in regulating eyespot number, placement, and integrity.

Original languageEnglish (US)
Pages (from-to)489-498
Number of pages10
JournalG3: Genes, Genomes, Genetics
Volume1
Issue number6
DOIs
StatePublished - Nov 2011

Fingerprint

Chlamydomonas reinhardtii
Mutation
Chlorophyta
Aptitude
Genetic Loci
Rhodopsin
Organelle Biogenesis
Chloroplasts
Cell Membrane
Proteins
Phototaxis

Keywords

  • Eyespot
  • MIN2
  • MLT2
  • Organelle biogenesis
  • Photoreception

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)

Cite this

Miniature-and multiple-eyespot loci in chlamydomonas reinhardtii define new modulators of eyespot photoreception and assembly. / Boyd, Joseph S.; Lamb, Mary Rose; Dieckmann, Carol L.

In: G3: Genes, Genomes, Genetics, Vol. 1, No. 6, 11.2011, p. 489-498.

Research output: Contribution to journalArticle

@article{382d077c86d34826a5fc9aa7497401e9,
title = "Miniature-and multiple-eyespot loci in chlamydomonas reinhardtii define new modulators of eyespot photoreception and assembly",
abstract = "The photosensory eyespot of the green alga Chlamydomonas reinhardtii is a model system for the study of organelle biogenesis and placement. Eyespot assembly and positioning are governed by several genetic loci that have been identified in forward genetic screens for phototaxis-defective mutants. These include the previously described miniature-eyespot mutant min1, the multiple-eyespot mutant mlt1, the eyeless mutants eye2 and eye3, and two previously uncharacterized eyespot mutants, min2 and mlt2. In this study, effects of miniature-and multiple-eyespot mutations and their combinations on the localization and expression levels of the rhodopsin photoreceptor channelrhodopsin-1 (ChR1) and the localization of the eyespot-assembly proteins EYE2 and EYE3 were examined. min2 mutants assemble a properly organized, albeit nonfunctional, eyespot that is slightly smaller than wild-type; however, combination of the min2 and mlt1 mutations resulted in drastic reduction of photoreceptor levels. Both stationary-phase mlt1 and mlt2 cells have supernumerary, mislocalized eyespots that exhibit partial or total dissociation of the eyespot layers. In these mutant strains, photoreceptor patches in the plasma membrane were never associated with pigment granule arrays in the chloroplast stroma unless EYE2 was present in the intervening envelope. The data suggest that MIN2 is required for the photoreceptive ability of the eyespot and that MLT2 plays a major role in regulating eyespot number, placement, and integrity.",
keywords = "Eyespot, MIN2, MLT2, Organelle biogenesis, Photoreception",
author = "Boyd, {Joseph S.} and Lamb, {Mary Rose} and Dieckmann, {Carol L}",
year = "2011",
month = "11",
doi = "10.1534/g3.111.000679",
language = "English (US)",
volume = "1",
pages = "489--498",
journal = "G3 (Bethesda, Md.)",
issn = "2160-1836",
publisher = "Genetics Society of America",
number = "6",

}

TY - JOUR

T1 - Miniature-and multiple-eyespot loci in chlamydomonas reinhardtii define new modulators of eyespot photoreception and assembly

AU - Boyd, Joseph S.

AU - Lamb, Mary Rose

AU - Dieckmann, Carol L

PY - 2011/11

Y1 - 2011/11

N2 - The photosensory eyespot of the green alga Chlamydomonas reinhardtii is a model system for the study of organelle biogenesis and placement. Eyespot assembly and positioning are governed by several genetic loci that have been identified in forward genetic screens for phototaxis-defective mutants. These include the previously described miniature-eyespot mutant min1, the multiple-eyespot mutant mlt1, the eyeless mutants eye2 and eye3, and two previously uncharacterized eyespot mutants, min2 and mlt2. In this study, effects of miniature-and multiple-eyespot mutations and their combinations on the localization and expression levels of the rhodopsin photoreceptor channelrhodopsin-1 (ChR1) and the localization of the eyespot-assembly proteins EYE2 and EYE3 were examined. min2 mutants assemble a properly organized, albeit nonfunctional, eyespot that is slightly smaller than wild-type; however, combination of the min2 and mlt1 mutations resulted in drastic reduction of photoreceptor levels. Both stationary-phase mlt1 and mlt2 cells have supernumerary, mislocalized eyespots that exhibit partial or total dissociation of the eyespot layers. In these mutant strains, photoreceptor patches in the plasma membrane were never associated with pigment granule arrays in the chloroplast stroma unless EYE2 was present in the intervening envelope. The data suggest that MIN2 is required for the photoreceptive ability of the eyespot and that MLT2 plays a major role in regulating eyespot number, placement, and integrity.

AB - The photosensory eyespot of the green alga Chlamydomonas reinhardtii is a model system for the study of organelle biogenesis and placement. Eyespot assembly and positioning are governed by several genetic loci that have been identified in forward genetic screens for phototaxis-defective mutants. These include the previously described miniature-eyespot mutant min1, the multiple-eyespot mutant mlt1, the eyeless mutants eye2 and eye3, and two previously uncharacterized eyespot mutants, min2 and mlt2. In this study, effects of miniature-and multiple-eyespot mutations and their combinations on the localization and expression levels of the rhodopsin photoreceptor channelrhodopsin-1 (ChR1) and the localization of the eyespot-assembly proteins EYE2 and EYE3 were examined. min2 mutants assemble a properly organized, albeit nonfunctional, eyespot that is slightly smaller than wild-type; however, combination of the min2 and mlt1 mutations resulted in drastic reduction of photoreceptor levels. Both stationary-phase mlt1 and mlt2 cells have supernumerary, mislocalized eyespots that exhibit partial or total dissociation of the eyespot layers. In these mutant strains, photoreceptor patches in the plasma membrane were never associated with pigment granule arrays in the chloroplast stroma unless EYE2 was present in the intervening envelope. The data suggest that MIN2 is required for the photoreceptive ability of the eyespot and that MLT2 plays a major role in regulating eyespot number, placement, and integrity.

KW - Eyespot

KW - MIN2

KW - MLT2

KW - Organelle biogenesis

KW - Photoreception

UR - http://www.scopus.com/inward/record.url?scp=84871854246&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871854246&partnerID=8YFLogxK

U2 - 10.1534/g3.111.000679

DO - 10.1534/g3.111.000679

M3 - Article

C2 - 22384359

AN - SCOPUS:84871854246

VL - 1

SP - 489

EP - 498

JO - G3 (Bethesda, Md.)

JF - G3 (Bethesda, Md.)

SN - 2160-1836

IS - 6

ER -