Miniature objective lens with variable focus for confocal endomicroscopy

Minkyu Kim, Dongkyun Kang, Tao Wu, Nima Tabatabaei, Robert W. Carruth, Ramses V. Martinez, George M. Whitesides, Yoshikazu Nakajima, Guillermo J. Tearney

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Spectrally encoded confocal microscopy (SECM) is a reflectance confocal microscopy technology that can rapidly image large areas of luminal organs at microscopic resolution. One of the main challenges for large-area SECM imaging in vivo is maintaining the same imaging depth within the tissue when patient motion and tissue surface irregularity are present. In this paper, we report the development of a miniature vari-focal objective lens that can be used in an SECM endoscopic probe to conduct adaptive focusing and to maintain the same imaging depth during in vivo imaging. The vari-focal objective lens is composed of an aspheric singlet with an NA of 0.5, a miniature water chamber, and a thin elastic membrane. The water volume within the chamber was changed to control curvature of the elastic membrane, which subsequently altered the position of the SECM focus. The vari-focal objective lens has a diameter of 5 mm and thickness of 4 mm. A vari-focal range of 240 μm was achieved while maintaining lateral resolution better than 2.6 μm and axial resolution better than 26 μm. Volumetric SECM images of swine esophageal tissues were obtained over the vari-focal range of 260 μm. SECM images clearly visualized cellular features of the swine esophagus at all focal depths, including basal cell nuclei, papillae, and lamina propria.

Original languageEnglish (US)
Article numberA4350
Pages (from-to)4350-4361
Number of pages12
JournalBiomedical Optics Express
Volume5
Issue number12
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Confocal Microscopy
Lenses
lenses
microscopy
swine
Swine
chambers
papillae
membranes
esophagus
Membranes
Water
Basal Ganglia
Cell Nucleus
irregularities
organs
water
Esophagus
Mucous Membrane
curvature

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Kim, M., Kang, D., Wu, T., Tabatabaei, N., Carruth, R. W., Martinez, R. V., ... Tearney, G. J. (2014). Miniature objective lens with variable focus for confocal endomicroscopy. Biomedical Optics Express, 5(12), 4350-4361. [A4350]. https://doi.org/10.1364/BOE.5.004350

Miniature objective lens with variable focus for confocal endomicroscopy. / Kim, Minkyu; Kang, Dongkyun; Wu, Tao; Tabatabaei, Nima; Carruth, Robert W.; Martinez, Ramses V.; Whitesides, George M.; Nakajima, Yoshikazu; Tearney, Guillermo J.

In: Biomedical Optics Express, Vol. 5, No. 12, A4350, 01.01.2014, p. 4350-4361.

Research output: Contribution to journalArticle

Kim, M, Kang, D, Wu, T, Tabatabaei, N, Carruth, RW, Martinez, RV, Whitesides, GM, Nakajima, Y & Tearney, GJ 2014, 'Miniature objective lens with variable focus for confocal endomicroscopy', Biomedical Optics Express, vol. 5, no. 12, A4350, pp. 4350-4361. https://doi.org/10.1364/BOE.5.004350
Kim M, Kang D, Wu T, Tabatabaei N, Carruth RW, Martinez RV et al. Miniature objective lens with variable focus for confocal endomicroscopy. Biomedical Optics Express. 2014 Jan 1;5(12):4350-4361. A4350. https://doi.org/10.1364/BOE.5.004350
Kim, Minkyu ; Kang, Dongkyun ; Wu, Tao ; Tabatabaei, Nima ; Carruth, Robert W. ; Martinez, Ramses V. ; Whitesides, George M. ; Nakajima, Yoshikazu ; Tearney, Guillermo J. / Miniature objective lens with variable focus for confocal endomicroscopy. In: Biomedical Optics Express. 2014 ; Vol. 5, No. 12. pp. 4350-4361.
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